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Complement Factor H-related 2/CFHR2/CFHL2 Antibody

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Western Blot: Complement Factor H-related 2/CFHR2/CFHL2 Antibody [H00003080-D01P] - Western Blot analysis of CFHR2 expression in human liver.
Western Blot: Complement Factor H-related 2/CFHR2/CFHL2 Antibody [H00003080-D01P] - Western Blot analysis of CFHR2 expression in human liver.
Western Blot: Complement Factor H-related 2/CFHR2/CFHL2 Antibody [H00003080-D01P] - Analysis of CFHR2 expression in transfected 293T cell line by CFHR2 polyclonal antibody.Lane 1: CFHR2 transfected lysate(30.70 ...read more

Product Details

Summary
Product Discontinued
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    • Catalog Number
      H00003080-D01P
    • Availability
      Product Discontinued

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Complement Factor H-related 2/CFHR2/CFHL2 Antibody Summary

Immunogen
CFHR2 (NP_005657.1, 1 a.a. - 270 a.a.) full-length human protein. MWLLVSVILISRISSVGGEAMFCDFPKINHGILYDEEKYKPFSQVPTGEVFYYSCEYNFVSPSKSFWTRITCAEEGWSPTPKCLRLCFFPFVENGHSESSGQTHLEGDTVQIICNTGYRLQNNENNISCVERGWSTPPKCRSTISAEKCGPPPPIDNGDITSFLLSVYAPGSSVEYQCQNLYQLEGNNQITCRNGQWSEPPKCLDPCVISQEIMEKYNIKLKWTNQQKLYSRTGDIVEFVCKSGYHPTKSHSFRAMCQNGKLVYPSCEEK
Specificity
Reacts with complement factor H-related 2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CFHR2
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This antibody is reactive against tissue and transfected lysate in WB and as a detection antibody in ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Complement Factor H-related 2/CFHR2/CFHL2 Antibody

  • CFHL2
  • CFHL2FHR-2
  • CFHR2
  • Complement Factor Hrelated 2
  • Complement Factor H-related 2
  • factor H-related gene 2
  • FHR2
  • FHR2DDESK59
  • H factor (complement)-like 3
  • H factor-like 3
  • H factor-like protein 2
  • HFL3
  • HFL3complement factor H-related protein 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Complement Factor H was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Complement Factor H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4779) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(2 Reviews)
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Applications: WB, IHC
Complement Factor
H-related 1/CFHR1/CFH ...
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Complement Factor H-related 1/CFHR1/CFHL1 Antibody (442127)
(2 Publications)
Species: Hu
Applications: WB, IP
Complement Factor
H-related 5/CFHR5 Ant ...
NBP2-52441
Western Blot: Complement Factor H-related 5/CFHR5 Antibody (3A10A5) [NBP2-52441] - Analysis using CFHR5 mAb against HEK293 (1) and CFHR5 (AA: 344-569)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/Immunofluorescence: Complement Factor H-related 5/CFHR5 Antibody (3A10A5) [NBP2-52441] - Analysis of Hela cells using CFHR5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunohistochemistry: Complement Factor H-related 5/CFHR5 Antibody (3A10A5) [NBP2-52441] - Analysis of paraffin-embedded ovarian cancer tissues using CFHR5 mouse mAb with DAB staining.
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
Complement Factor
H-related 4/CFHR4 Ant ...
MAB5980
Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 2 µg/mL of Human Complement Factor H-related 4/CFHR4 Monoclonal Antibody (Catalog # MAB5980) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Complement Factor H‑related 4/CFHR4 at approximately 45 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
HepG2 human hepatocellular carcinoma cell line was stained with Human Complement Factor H-related 4/CFHR4 Monoclonal Antibody (Catalog # MAB5980, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with PFA and permeabilized with saponin.
Complement Factor H-related 4/CFHR4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMC) using Human Complement Factor H-related 4 Monoclonal Antibody (Catalog # MAB5980) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(1 Review)
(1 Publication)
Species: Hu
Applications: WB, CyTOF-ready, ICC, ICFlow
Complement Factor
H-related 3/CFHR3 Ant ...
NBP2-92615
Immunocytochemistry/Immunofluorescence: Complement Factor H-related 3/CFHR3 Antibody [NBP2-92615] - Analysis of A549 cells using Complement Factor H-related 3/CFHR3. Blue: DAPI for nuclear staining.
Species: Hu, Mu
Applications: WB, ICC/IF
Complement C3
Antibody (11H9)
NB200-540
Flow (Intracellular): Complement C3 Antibody (11H9) [NB200-540] - An intracellular stain was performed on RAW 246.7 cells with Complement C3 (11H9-3-2) antibody NB200-540 (blue) and a matched isotype control NBP2-31382 (orange). Cells were either treated with 3uM Monensin for 3 hours to block the secretion of Complement C3 (B) or grown in normal media (A). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).
Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (11H9) [NB200-540] - C3 protein fragments deposited on kidney cells of MPL-lpr mouse. Staining with antibody 11H9. Glomerular staining pattern. Fixation in 4% paraformaldehyde in PBS pH 7.4. Vibratome sections of 4 um. Pretreated with 3% hydrogen peroxide for 20 min to quench endogenous peroxidases. Microwaved in antigen unmasking solution for 2-5 minutes as antigen retrieval.
Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - Complement C3 protein in a FFPE tissue section of mouse liver using 1:100 dilution of Complement C3 antibody (clone 11H9) NB200-540. Weak but distinct membrane-cytoplasmic immunopositivity was observed in hepatocytes and few cells developed punctate membrane staining.
(2 Reviews)
(9 Publications)
Species: Mu
Applications: Flow, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
FHL1 Antibody
(767418)
MAB5938
Western blot shows lysates of human skeletal muscle tissue and human heart tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human FHL1 Monoclonal Antibody (Catalog # MAB5938) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FHL1 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FHL1 was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line using Mouse Anti-Human FHL1 Monoclonal Antibody (Catalog # MAB5938) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter­stained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Applications: WB, ICC
Complement Factor
I Antibody (OTI7C9) - ...
NBP2-70997
Western Blot: Complement Factor I Antibody (OTI7C9) - Azide and BSA Free [NBP2-70997] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Complement Factor 1.
Immunohistochemistry: Complement Factor I Antibody (OTI7C9) - Azide and BSA Free [NBP2-70997] - Analysis of Carcinoma of Human lung tissue, dilution 1:150
Immunohistochemistry: Complement Factor I Antibody (OTI7C9) - Azide and BSA Free [NBP2-70997] - Analysis of Adenocarcinoma of Human ovary tissue, dilution 1:150
Species: Hu
Applications: WB, IHC
Apolipoprotein
E/ApoE Antibody (WUE-
NB110-60531
Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE was detected in immersion fixed paraffin-embedded sections of human liver using anti-human mouse monoclonal antibody (Catalog # NB110-60531) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
Western Blot: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE Antibody (WUE-4) [NB110-60531] - Detection of ApoE in human tissue lysate using NB110-60531. Lane 1: liver Lane 2: brain
Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE Antibody (WUE-4) [NB110-60531] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using ApoE antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
(2 Reviews)
(21 Publications)
Species: Hu, Mu, Rt(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready, Flow-IC
FBXO3 Antibody
NBP1-55046
Western Blot: FBXO3 Antibody [NBP1-55046] - Sample Type: Human Adult Placenta Antibody Dilution: 1.0 ug/ml
Western Blot: FBXO3 Antibody [NBP1-55046] - Sample Type: Human Fetal Liver Antibody Dilution: 1.0 ug/ml
Western Blot: FBXO3 Antibody [NBP1-55046] - Sample Type: Human Fetal Heart Antibody Dilution: 1.0 ug/ml
Species: Hu
Applications: WB
NGFI-B
alpha/Nur77/NR4A1 Ant
NB100-56745
Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - Analysis of Nur77 (Nak-1) in 15 ugs of NIH3T3 cell lysate using Nur77 antibody at 1:500 dilution
Immunocytochemistry/Immunofluorescence: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-NGFI-B alpha at 5 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunocytochemistry/Immunofluorescence: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - Antibody tested in Jurkat cells at a 1:50 dilution against DyLight 488 (Green). Alpha tubulin and nuclei were counterstained against DyLight 568 (Red) and DAPI (Blue), respectively.
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c-Myc Antibody
NB600-335
Western Blot: c-Myc Antibody [NB600-335] - Detection of c-myc-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-c-myc antibody NB600-335 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunocytochemistry/Immunofluorescence: c-Myc Antibody [NB600-335] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-c-Myc conjugated to Alexa Fluor 488 [NB600-335AF488] at 20ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image using the Alexa Fluor 488 format of this antibody.
Western Blot: c-Myc Antibody [NB600-335] - Analysis using the HRP conjugate of NB600-335. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.4 ug/ml (1:25,000).
(1 Review)
(49 Publications)
Species: Hu, Mu, Ec, All-NA
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
EMMPRIN/CD147
Antibody [Unconjugate
AF972
Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for EMMPRIN/CD147 at approximately38-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
EMMPRIN/CD147 was detected in immersion fixed paraffin-embedded sections of human cervix using Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western blot shows lysates of HEK293T human embryonic kidney parental cell line and EMMPRIN/CD147 knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EMMPRIN/CD147 at approximately 51 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(1 Review)
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MAP2 Antibody
NB300-213
Immunocytochemistry/Immunofluorescence: MAP2 Antibody [NB300-213] - Mouse primary cultures stained with MAP2 antibody. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: MAP2 Antibody [NB300-213] - Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to microtubule associated protein 2 (MAP2), NB300-213, dilution 1:10,000 in red, and mouse mAb to MAP-tau, NPB2-25162, dilution 1:2,000, in green. The blue is DAPI staining of nuclear DNA. NB300-213 antibody stains dendrites and perikarya of neurons, while NBP2-25162 antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green.
Western Blot: MAP2 Antibody [NB300-213] - Western blot analysis of whole brain tissue lysates using chicken pAb to microtubule associated protein 2 (MAP2), NB300-213, dilution 1:50,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain, [4] adult mouse brain. Strong band observed at ~280kDa mark corresponds to two major isoforms of MAP2 protein referred to as MAP2A and MAP2B. Smaller fragments of these isoforms are also detected if the antibody is used at higher concentrations.
(4 Reviews)
(59 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, KD
NR4A3/NOR1
Antibody (OTI5C2)
NBP2-46246
Western Blot: NR4A3/NOR1 Antibody (5C2) [NBP2-46246] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR4A3.
Immunohistochemistry-Paraffin: NR4A3/NOR1 Antibody (5C2) [NBP2-46246] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0) Dilution: 1:150
Immunohistochemistry: NR4A3/NOR1 Antibody (5C2) [NBP2-46246] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0) Dilution: 1:150
(1 Publication)
Species: Hu
Applications: WB, IHC, IHC-P
ATP8A2 Antibody
NBP2-55197
Immunocytochemistry/Immunofluorescence: ATP8A2 Antibody [NBP2-55197] - Staining of human cell line PC-3 shows localization to plasma membrane.
Species: Hu
Applications: ICC/IF
Integrin beta
2/CD18 Antibody [Unco ...
AF1730
Integrin  beta 2/CD18 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human Integrin  beta 2/CD18 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1730) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
(9 Publications)
Species: Hu
Applications: Flow, AdBlk, CyTOF-ready, ICC

Transthyretin/Prealbum ...
NBP2-52575
Western Blot: Transthyretin/Prealbumin Antibody (2E10C5) [NBP2-52575] - Analysis using TTR mAb against human TTR (AA: 1-147) recombinant protein. (Expected MW is 45.8 kDa)
Immunocytochemistry/Immunofluorescence: Transthyretin/Prealbumin Antibody (2E10C5) [NBP2-52575] - Analysis of MCF-7 cells using TTR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunohistochemistry-Paraffin: Transthyretin/Prealbumin Antibody (2E10C5) [NBP2-52575] - Analysis of liver cancer tissues using TTR mouse mAb with DAB staining.
(1 Publication)
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready

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Bioinformatics

Gene Symbol CFHR2
Entrez
Uniprot