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Complement Factor H-related 2/CFHR2/CFHL2 Antibody

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Western Blot: Complement Factor H-related 2/CFHR2/CFHL2 Antibody [H00003080-D01P] - Western Blot analysis of CFHR2 expression in human liver.
Western Blot: Complement Factor H-related 2/CFHR2/CFHL2 Antibody [H00003080-D01P] - Western Blot analysis of CFHR2 expression in human liver.
Western Blot: Complement Factor H-related 2/CFHR2/CFHL2 Antibody [H00003080-D01P] - Analysis of CFHR2 expression in transfected 293T cell line by CFHR2 polyclonal antibody.Lane 1: CFHR2 transfected lysate(30.70 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Complement Factor H-related 2/CFHR2/CFHL2 Antibody Summary

Immunogen
CFHR2 (NP_005657.1, 1 a.a. - 270 a.a.) full-length human protein. MWLLVSVILISRISSVGGEAMFCDFPKINHGILYDEEKYKPFSQVPTGEVFYYSCEYNFVSPSKSFWTRITCAEEGWSPTPKCLRLCFFPFVENGHSESSGQTHLEGDTVQIICNTGYRLQNNENNISCVERGWSTPPKCRSTISAEKCGPPPPIDNGDITSFLLSVYAPGSSVEYQCQNLYQLEGNNQITCRNGQWSEPPKCLDPCVISQEIMEKYNIKLKWTNQQKLYSRTGDIVEFVCKSGYHPTKSHSFRAMCQNGKLVYPSCEEK
Specificity
Reacts with complement factor H-related 2.
Clonality
Polyclonal
Host
Rabbit
Gene
CFHR2
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This antibody is reactive against tissue and transfected lysate in WB and as a detection antibody in ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Complement Factor H-related 2/CFHR2/CFHL2 Antibody

  • CFHL2
  • CFHL2FHR-2
  • CFHR2
  • Complement Factor Hrelated 2
  • Complement Factor H-related 2
  • factor H-related gene 2
  • FHR2
  • FHR2DDESK59
  • H factor (complement)-like 3
  • H factor-like 3
  • H factor-like protein 2
  • HFL3
  • HFL3complement factor H-related protein 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western blot shows lysates of Hepa 1‑6 mouse hepatoma cell line and mouse plasma. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Complement Factor H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4999) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Complement Factor H at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows mouse plasma, loaded at 0.2 mg/mL. A specific band was detected for Complement Factor H at approximately 166 kDa (as indicated) using 10 µg/mL of Sheep Anti-Mouse Complement Factor H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4999) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
Species: Mu
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(2 Publications)
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Complement Factor
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Western Blot: Complement Factor H-related 5/CFHR5 Antibody (3E1E10) [NBP2-52440] - Analysis using CFHR5 mAb against human CFHR5 (AA: 344-569) recombinant protein. (Expected MW is 51.8 kDa)
Immunohistochemistry: Complement Factor H-related 5/CFHR5 Antibody (3E1E10) [NBP2-52440] - Analysis of paraffin-embedded endometrial cancer tissues using CFHR5 mouse mAb with DAB staining.
Flow Cytometry: Complement Factor H-related 5/CFHR5 Antibody (3E1E10) [NBP2-52440] - Flow cytometric analysis of Hela cells using CFHR5 mouse mAb (green) and negative control (red).
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Complement Factor
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Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 2 µg/mL of Human Complement Factor H-related 4/CFHR4 Monoclonal Antibody (Catalog # MAB5980) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Complement Factor H‑related 4/CFHR4 at approximately 45 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
HepG2 human hepatocellular carcinoma cell line was stained with Human Complement Factor H-related 4/CFHR4 Monoclonal Antibody (Catalog # MAB5980, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with PFA and permeabilized with saponin.
Complement Factor H-related 4/CFHR4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMC) using Human Complement Factor H-related 4 Monoclonal Antibody (Catalog # MAB5980) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(1 Review)
(1 Publication)
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Applications: WB, CyTOF-ready, ICC, ICFlow
Complement C3
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NB200-540
Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (11H9) [NB200-540] - C3 protein fragments deposited on kidney cells of MPL-lpr mouse. Staining with antibody 11H9. Glomerular staining pattern.
Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - IHC-P detection of Complement C3 protein in a formalin fixed paraffin embedded tissue section of mouse liver using 1 : 100 dilution of Complement C3 antibody (clone 11H9) NB200-540. Weak but distinct membrane-cytoplasmic immunopositivity was observed in hepatocytes and few cells developed punctate membrane staining.
Flow (Intracellular): Complement C3 Antibody (11H9) [NB200-540] - An intracellular stain was performed on RAW 246.7 cells with Complement C3 (11H9-3-2) antibody NB200-540 (blue) and a matched isotype control NBP2-31382 (orange). Cells were either treated with 3uM Monensin for 3 hours to block the secretion of Complement C3 (B) or grown in normal media (A). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).
(1 Review)
(1 Publication)
Species: Mu
Applications: WB, Flow, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready
FHL1 Antibody (2A9)
H00002273-M01
Western Blot: FHL1 Antibody (2A9) [H00002273-M01] - FHL1 monoclonal antibody (M01), clone 2A9. Analysis of FHL1 expression in NIH/3T3.
Western Blot: FHL1 Antibody (2A9) [H00002273-M01] - FHL1 monoclonal antibody (M01), clone 2A9. Analysis of FHL1 expression in PC-12.
Western Blot: FHL1 Antibody (2A9) [H00002273-M01] - FHL1 monoclonal antibody (M01), clone 2A9. Analysis of FHL1 expression in Raw 264.7.
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, ELISA
Complement Factor
I Antibody (271203)
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Complement Factor I Antibody (271203)
(1 Publication)
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Applications: WB, IP
Apolipoprotein
E/ApoE Antibody (WUE-
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Western Blot: ApoE Antibody (WUE-4) [NB110-60531] - 50 ug protein per lane for liver lysate and 0.5 ul of plasma at 9% SDS. Samples are loaded onto the gel as the following order: human liver lysate, mouse liver lysate, rat liver lysate, human plasma, and mouse plasma. Image from confirmed customer review.
Immunocytochemistry/Immunofluorescence: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-ApoE (WUE-4) [NB110-60531] at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE was detected in immersion fixed paraffin-embedded sections of human liver using anti-human mouse monoclonal antibody (Catalog # NB110-60531) at 1:200 dilution overnight at 4 C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
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FBXO3 Antibody
NBP1-55046
Western Blot: FBXO3 Antibody [NBP1-55046] - Sample Type: Human Adult Placenta Antibody Dilution: 1.0 ug/ml
Western Blot: FBXO3 Antibody [NBP1-55046] - Sample Type: Human Fetal Liver Antibody Dilution: 1.0 ug/ml
Western Blot: FBXO3 Antibody [NBP1-55046] - Sample Type: Human Fetal Heart Antibody Dilution: 1.0 ug/ml
Species: Hu
Applications: WB
NGFI-B
alpha/Nur77/NR4A1 Ant
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Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - Total protein from Human and Mouse brain using a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NGFI-B alpha in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
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Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - Analysis of Nur77 (Nak-1) in 15 ugs of NIH3T3 cell lysate using Nur77 antibody at 1:500 dilution
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EMMPRIN/CD147
Antibody [Unconjugate
AF972
Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for EMMPRIN/CD147 at approximately38-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
EMMPRIN/CD147 was detected in immersion fixed paraffin-embedded sections of human cervix using Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for EMMPRIN/CD147 at approximately 70 kDa (as indicated) using 10 µg/mL of Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.        
(1 Review)
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MAP2 Antibody
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Western Blot: MAP2 Antibody [NB300-213] - Western blot analysis of MAP2 expression in bovine brain extract using NB300-213.
Immunocytochemistry/Immunofluorescence: MAP2 Antibody [NB300-213] - Mouse primary cultures stained with MAP2 antibody. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: MAP2 Antibody [NB300-213] - Mixed neuron/glial cultures stained with NB300-213 (red). The perikarya and dendrites of neurons are strongly and specifically stained with the MAP2 antibody, while the axons of the neurons and the processes of all other cell types in these cultures (astrocytes, oligodendrocytes, microglia, endothelia and fibroblasts) are all negative. Cell nuclei are visualized with DAPI DNA stain..
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Applications: WB, ICC/IF, IHC, IHC-Fr
NGFI-B
gamma/NOR-1/NR4A3 Ant ...
PP-H7833-00
NGFI-B gamma/NOR-1/NR4A3 Antibody (H7833)
(8 Publications)
Species: Hu
Applications: WB, IP
ATP8A2 Antibody
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Immunohistochemistry-Paraffin: ATP8A2 Antibody [NBP1-88899] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.
Species: Hu
Applications: IHC, IHC-P
Integrin beta
2/CD18 Antibody (YFC1 ...
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Immunocytochemistry/Immunofluorescence: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - A-D Confocal microscopy of IBA-1 (green staining) immunohistochemistry of RPE flatmounts (RPE autofluorescence visible as orange due to its autofluorescence in the red and green channel) from a healthy donor (A), a geographic atrophy lesion (B), and large drusen (C and D). (A, B, D): orthogonal Z-stack projection; (C): oblique Z-stack projection and dissecting microscope appearance of postmortem large drusen after removal of the overlaying retina (inset). E-G Double-labeling on the subretinal side of the retina (to avoid masking by RPE autofluorescence) of IBA-1+ (E, green fluorescence) and CD18 (F, red fluorescence; G, merge). H. Orthogonal and lateral Z-stack of a subretinal IBA-1+ (green fluorescence) MPs adjacent to the RPE (orange autofluorescence) in the vicinity of a large drusen.
Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Analysis using the Biotin conjugate of NB200-610. Staining of human peripheral blood lymphocytes.
Immunocytochemistry/Immunofluorescence: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Analysis of canine thoracic fluid samples using anti-Integrin beta 2 antibody. The antibody showing positive cell surface staining. Image from verified customer review.
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Transthyretin/Prealbum
NBP1-89649
Western Blot: Transthyretin/Prealbumin Antibody [NBP1-89649] - Analysis in human plasma.
Immunohistochemistry-Paraffin: Transthyretin/Prealbumin Antibody [NBP1-89649] - Immunohistochemical staining of human tonsil shows no positivity as expected.
Immunohistochemistry-Paraffin: Transthyretin/Prealbumin Antibody [NBP1-89649] - Immunohistochemical staining of human duodenum shows positivity in plasma.
(3 Publications)
Species: Hu
Applications: WB, IHC, IHC-P
Complement Factor
H-related 2/CFHR2/CFH ...
H00003080-D01P
Complement Factor H-related 2/CFHR2/CFHL2 Antibody
Species: Hu
Applications: WB

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Bioinformatics

Gene Symbol CFHR2
Entrez
Uniprot

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