Complement Component C9 Antibody (22) - BSA Free

Immunohistochemistry: Complement Component C9 Antibody (22) - BSA Free [NBP2-21612] - Staining of human liver with complement component C9 antibody. Plasma of portal veins are strongly stained.

Immunohistochemistry: Complement Component C9 Antibody (22) - BSA Free [NBP2-21612] - Staining of human kidney with complement component C9 antibody. Plasma of veins are strongly stained.

Immunohistochemistry: Complement Component C9 Antibody (22) - BSA Free [NBP2-21612] - staining of tonsil tissue with complement component C9 antibody. Follicular dendritic cell network stained strongly in germinal centers of tonsil tissue.

Immunohistochemistry: Complement Component C9 Antibody (22) - BSA Free [NBP2-21612] - staining of tonsil tissue with complement component C9 antibody. Follicular dendritic cell network stained strongly in germinal centers of tonsil tissue. Also moderate staining were observed from plasma of veins.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 22
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Complement Component C9 Antibody (22) - BSA Free Summary

• Immunogen: Complement factor C9
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: C9
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: ELISA: NBP2-21612 recognizes C9 in human serum diluted 1/50 in Tris buffer (20 mM Tris-base, 1 mM MgCl2, 1 mM CaCl2 and 140 mM NaCl) and incubated for 2 hours at 37C using a human IgM coated (10 ug/mL overnight at 4C, blocked with PBS 7.2 + 1% BSA for 1 hour) ELISA plate.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Complement Component C9 Antibody (22) - BSA Free

• complement component 9
• Complement Component C9

Background

Complement factor C9 is a 71kDa single-chain ellipsoid a-globular molecule of 558 amino acids including a leader sequence of 20 amino acids. C9 bind the C5b8 complex on membranes and has an unique property in forming tubular structures containing between 12 and 18 monomers, closely resembling the membrane attack complex (MAC). The primary site of synthesis is hepatocytes, secondary sites are monocytes, fibroblasts and glial cells. The normal level of C9 in plasma is 60 ug/mL, and the level is up regulated from hepatocytes by IFN-gamma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: C9