Complement Component C5a Antibody (295103) [Alexa Fluor® 750]

• Applications: WB
• Clone: 295103
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 750

Complement Component C5a Antibody (295103) [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant mouse C5a
  Asn679-Arg755
  Accession # P06684
• Specificity: Detects mouse Complement Component C5a in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross‑react with recombinant human (rh) C3a, rmC3d, or rhC5a.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: C5
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Mouse Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as Anaphylatoxins. C5a is a 77 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain (1, 2). Mouse C5a has four alpha -helices, plus three intrachain disulfide bonds that create a triple loop structure (3). In serum, proteolytic processing removes the C-terminal arginine, creating a low activity C5a desArg77 molecule (1). Mouse C5a shares 60% and 82% aa sequence identity to human and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (GPCR) (C5aR/CD88), inducing neutrophil chemotaxis and endothelial cell activation (1, 4). It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells (1, 4). Alternatively, it may also bind to a nonsignaling GPCR termed C5L2 whose function is yet to be determined (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: C5