Complement Component C5/C5a Antibody (295009) [Unconjugated]

Recombinant Human Complement Component C5/C5a protein was serially diluted 2-fold and captured by Mouse Anti-Human Complement Component C5/C5a Monoclonal Antibody (Catalog # MAB2037) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Complement Component C5/C5a Monoclonal Antibody (Catalog # MAB20371) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

• Reactivity: Hu
• Applications: ELISA
• Clone: 295009
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Complement Component C5/C5a Antibody (295009) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Complement Component C5a
  Thr678-Arg751
  Accession # P01031
• Specificity: Detects human Complement Component C5/C5a in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Complement Component C5/C5a Antibody (295009) [Unconjugated]

• C5
• C5a;C5b;C5D;Complement C5;CPAMD4;ECLZB
• Complement Component C5/C5a

Background

Human complement component C5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain (1, 2). Human C5a has four alpha -helices plus three intrachain disulfide bonds that create a triple loop structure (3). In serum, proteolytic processing removes the C-terminal arginine, creating a low activity C5a desArg74 molecule (1). Human C5a is 60% and 54% aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a non-signaling GPCR termed C5L2 (4). Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation (1, 5). It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.

1. Gerard, C. and N.P. Gerard (1994) Annu. Rev. Immunol. 12:775.
2. DiScipio, R.G. et al. (1983) J. Biol. Chem. 258:10629.
3. Huber-Lang, M.S. et al. (2003) J. Immunol. 170:6115.
4. Okinaga, S. et al. (2003) Biochemistry 42:9406.
5. Gerard, N.P. and C. Gerard (2002) Curr. Opin. Immunol. 14:705.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P01031()