Collagen VII Antibody (LH7.2) - BSA Free

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: LH7.2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Collagen VII Antibody (LH7.2) - BSA Free Summary

• Immunogen: Cells from a single cell suspension of epidermal cells (obtained from fresh human neonatal foreskin) were lysed with Nonidet P40 in phosphate buffered saline and the insoluble pellet was sonicated to prepare insoluble fractions.
• Epitope: The epitope is on the NC1 domain.
• Localization: Secreted, extracellular space, extracellular matrix, basement membrane.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: COL7A1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Theoretical MW: 250 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Collagen VII Antibody (LH7.2) - BSA Free

• Collagen 7
• collagen alpha-1(VII) chain
• collagen VII, alpha-1 polypeptide
• collagen, type VII, alpha 1
• Collagen-7
• EBD1
• EBDCT
• EBR1
• epidermolysis bullosa, dystrophic, dominant and recessive
• LC collagen
• long chain collagen
• Long-chain collagen

Background

Type VII collagen fibrils are composed of three identical alpha collagen chains. It's location is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in the collagen V11 gene are associated with all forms of dystrophic epidermolysis bullosa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: COL7A1
• Entrez:
  • 1294(Human)