Collagen V Antibody

Images

 
Immunohistochemistry-Paraffin: Collagen V Antibody [NBP1-26551] - Human gastric cancer tissue was stained with Goat Anti-Type V Collagen-UNLB followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP.
Immunohistochemistry-Frozen: Collagen V Antibody [NBP1-26551] - Frozen newborn mouse cartilage section was stained with Goat Anti-Type V Collagen-UNLB followed by Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-AF555 and ...read more
Immunohistochemistry-Frozen: Collagen V Antibody [NBP1-26551] - Frozen D-arginine treated Mdx mouse quadricep muscle section was stained with Goat Anti-Type V Collagen-UNLB followed by a secondary antibody.Image from ...read more
Immunohistochemistry-Frozen: Collagen V Antibody [NBP1-26551] - Frozen newborn mouse cartilage section was stained with Goat Anti-Type V Collagen-UNLB followed by Mouse Anti-Goat IgG Fc-AF555 and DAPI.
Immunohistochemistry-Paraffin: Collagen V Antibody [NBP1-26551] - Paraffin embedded mouse inguinal mammary glands were stained with Goat Anti-Type V Collagen-UNLB followed by a secondary antibody and DAPI. Images from ...read more
Immunohistochemistry-Frozen: Collagen V Antibody [NBP1-26551] - Frozen newborn mouse cartilage section was stained with Goat Anti-Type V Collagen-UNLB followed by Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-AF555 ...read more

Product Details

Summary
Reactivity Hu, Bv, EqSpecies Glossary
Applications WB, DB, ELISA, ICC/IF, IHC, IHC-Fr, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.4 mg/ml

Order Details

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Catalog# & Conjugate Size Price

Collagen V Antibody Summary

Immunogen
Goats were hyperimmunized with human type V collagen.
Specificity
Reacts with conformational determinants on human type V collagen as demonstrated by ELISA. May react with type V collagen from other species. Exhibits <10% cross reactivity with collagen type I, II, III, IV and VI. The antibody has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
COL5A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Dot Blot 1:100-1:2000
  • ELISA 1:1000-1:4000
  • Immunocytochemistry/Immunofluorescence 1:10-1:20
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:20-1:40
  • Immunocytochemistry
Positive Control
Collagen V Lysate (NBP2-65184)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.1M BBS (pH 8.2)
Preservative
No Preservative
Concentration
0.4 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Collagen V Antibody

  • alpha 1 type V collagen
  • Collagen 5
  • collagen alpha-1(V) chain
  • collagen, type V, alpha 1
  • Collagen-5
  • EC 2.7.7.6
  • EC 6.1.1

Background

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. Type V Collagen is a member of group I Collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V Collagen binds to DNA, heparan sulfate, Thrombospondin, Heparin, and Insulin. Collagen V is composed of several subunits as follows: trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta. Type V Collagen is closely related to type XI Collagen and it is possible that the Collagen chains of types V and XI constitute a single collagen type with tissue specific chain combinations. Mutations in this gene are associated with Ehlers Danlos syndrome, types I and II.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol COL5A1