Collagen V Antibody

Product Discontinued

Collagen V Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 300-350 of Human COL5A1.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: COL5A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of COL5A1 (E325) pAb in extracts from HeLa cells.
  Immunohistochemistry (IHC) analyzes of COL5A1 (E325) pAb in paraffin-embedded human heart tissue. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24800655). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 26220971)
• Theoretical MW: 180 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Collagen V Antibody

• alpha 1 type V collagen
• Collagen 5
• collagen alpha-1(V) chain
• collagen, type V, alpha 1
• Collagen-5
• EC 2.7.7.6
• EC 6.1.1

Background

The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Collagen V Antibody (NBP1-19633)(6)

Publications using NBP1-19633

• Zaffiri L, Shah RJ, Stearman RS et al. Collagen type-V is a danger signal associated with primary graft dysfunction in lung transplantation Transpl. Immunol. 2019-07-17 [PMID: 31325493] (FLOW, Mouse)
• Makuszewska M, Bonda T, Cieslinska M et al. Expression of collagens type I and V in healing rat's tympanic membrane Int. J. Pediatr. Otorhinolaryngol. 2018-12-18 [PMID: 30590281] (WB, Rat)
• Papke CL, Tsunezumi J, Ringuette LJ et al. Loss of fibulin-4 disrupts collagen synthesis and maturation: implications for pathology resulting from EFEMP2 mutations. Hum. Mol. Genet. 2015-07-28 [PMID: 26220971] (IHC-Fr, Mouse)
• Herchenhan A, Uhlenbrock F, Eliasson P et al. Lysyl Oxidase activity is required for ordered collagen fibrillogenesis by tendon cells. J. Biol. Chem. 2015-05-15 [PMID: 25979340] (WB, Human)
  
  Details:
  Tenascin X antibody was used at 1:500 dilution with ON 4C incubation for WB detection of established collagen fibrillogenesis regulator Tenascin X protein on samples from the control or beta-aminoproprionitrile /BAPN treated tendon constructs which were prepared using tendon fibroblasts isolated from human semitendinosus and gracilis tendon of human anterior cruciate ligament/ACL rupture patients undergoing reconstructive surgery. See the full text for protocol related details and the data is shown in FIGURE 7A.
• Karamichos D, Hutcheon AE, Zieske JD. Reversal of fibrosis by TGF-beta3 in a 3D in vitro model. Exp. Eye Res. 2014-05-04 [PMID: 24800655] (ICC/IF, Human)
• Karamichos D, Rich CB, Zareian R et al. TGF-beta3 Stimulates Stromal Matrix Assembly by Human Corneal Keratocyte-Like Cells. Invest Ophthalmol Vis Sci. 2013-10-09 [PMID: 24022012]

Bioinformatics

• Gene Symbol: COL5A1
• Entrez:
  • 1289(Human)
• Uniprot:
  • P20908(Human)