Collagen V Antibody

Images

 
Western Blot: Collagen V Antibody [NBP1-19633] - Extracts from HeLa cells.
Immunohistochemistry-Paraffin: Collagen V Antibody [NBP1-19633] - Paraffin-embedded human colorectal carcinoma tissue at 1:50.
Western Blot: Collagen V Antibody [NBP1-19633] - Lane1:Jurkat whole cell lysate. Lane2:Mouse liver tissue lysate. Lane3:Rat liver tissue lysate.
Immunohistochemistry-Paraffin: Collagen V Antibody [NBP1-19633] - Paraffin-embedded human heart tissue.

Product Details

Summary
Product Discontinued
View other related Collagen V Primary Antibodies

Order Details


    • Catalog Number
      NBP1-19633
    • Availability
      Product Discontinued

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Collagen V Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 300-350 of Human COL5A1.
Clonality
Polyclonal
Host
Rabbit
Gene
COL5A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of COL5A1 (E325) pAb in extracts from HeLa cells.
Immunohistochemistry (IHC) analyzes of COL5A1 (E325) pAb in paraffin-embedded human heart tissue. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24800655). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 26220971)
Theoretical MW
180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-19633 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Collagen V Antibody

  • alpha 1 type V collagen
  • Collagen 5
  • collagen alpha-1(V) chain
  • collagen, type V, alpha 1
  • Collagen-5
  • EC 2.7.7.6
  • EC 6.1.1

Background

The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Collagen V Antibody (NBP1-19633)(4)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IHC-Fr, WB.


Filter By Application
ICC/IF
(1)
IHC-Fr
(1)
WB
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
All Species
Showing Publications 1 - 4 of 4.
Publications using NBP1-19633 Applications Species
Papke CL, Tsunezumi J, Ringuette LJ et al. Loss of fibulin-4 disrupts collagen synthesis and maturation: implications for pathology resulting from EFEMP2 mutations. Hum. Mol. Genet. 2015 Jul 28 [PMID: 26220971] (IHC-Fr, Mouse) IHC-Fr Mouse
Herchenhan A, Uhlenbrock F, Eliasson P et al. Lysyl Oxidase activity is required for ordered collagen fibrillogenesis by tendon cells. J. Biol. Chem. 2015 May 15 [PMID: 25979340] (WB, Human)

Details:
Collagen V antibody was used at 1:500 dilution with ON 4C incubation for WB detection of established collagen fibrillogenesis regulator Collagen V protein in samples from the control or beta-aminoproprionitrile /BAPN treated tendon constructs which were prepared using tendon fibroblasts isolated from human semitendinosus and gracilis tendon of human anterior cruciate ligament/ACL rupture patients undergoing reconstructive surgery. See the full text for protocol related details and the data is shown in FIGURE 7B. Splice or processing variants of the collagen pN alpha 1(V) chain were observed (similarly positioned) in control as well as BAPN-treated samples and only low levels of dimers were found.
WB Human
Karamichos D, Hutcheon AE, Zieske JD. Reversal of fibrosis by TGF-beta3 in a 3D in vitro model. Exp. Eye Res. 2014 May 04 [PMID: 24800655] (ICC/IF, Human) ICC/IF Human
Karamichos D, Rich CB, Zareian R et al. TGF-beta3 Stimulates Stromal Matrix Assembly by Human Corneal Keratocyte-Like Cells. Invest Ophthalmol Vis Sci. 2013 Oct 9 [PMID: 24022012]

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol COL5A1
Entrez
Uniprot