Collagen V alpha 2 Overexpression Lysate (Native) (NBL1-09355)

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Western Blot: Collagen V alpha 2 Overexpression Lysate (Adult Normal) [NBL1-09355] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Collagen V alpha 2.

Product Details

Summary
Applications WB

Order Details

Collagen V alpha 2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC213335 and based on accession number NM_000393. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens collagen, type V, alpha 2 (COL5A2), mRNA.
Gene
COL5A2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
144.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Collagen V alpha 2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Collagen V alpha 2 Overexpression Lysate (Native)

  • AB collagen
  • collagen alpha-2(V) chain
  • collagen, fetal membrane, A polypeptide
  • collagen, type V, alpha 2
  • MGC105115
  • type V preprocollagen alpha 2 chain

Background

This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Collagen V alpha 2 Lysate (NBL1-09355) (0)

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Bioinformatics

Gene Symbol COL5A2