Collagen IV Antibody - BSA Free

Product Discontinued

Collagen IV Antibody - BSA Free Summary

• Immunogen: Collagen IV purified from mouse EHS tumour
• Specificity: NB110-59981 is specific for mouse collagen type IV. The following cross reactivities have been observed: Mouse Type IV (100%), Mouse Type III (<0.1%), Mouse Type II (<0.1%), Mouse Type I (<0.1%), Human Type IV (<0.1%), Human Type V (<0.1%), Mouse fibronectin (<0.1%), Mouse laminin (<0.1%).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: COL4A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:400
  • Immunocytochemistry/ Immunofluorescence 1:40-1:60
  • Immunohistochemistry 1:100 - 1:400
  • Immunohistochemistry-Frozen 1:500
  • Immunohistochemistry-Paraffin 1:250-1:1000
• Application Notes: IHC on paraffin embedded sections antigen retieval is not necessary but may imporve results.

Reactivity Notes

Orangutan, Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Collagen IV Antibody - BSA Free

• BSVD;BSVD1;COL4A1s;Collagen alpha-1(IV) chain;PADMAL;RATOR
• Collagen IV

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Collagen IV Antibody (NB110-59981)(5)

Publications using NB110-59981

• Coeyman SJ, Zhang Y, Baicu CF et al. In vitro bioreactor for mechanical control and characterization of tissue constructs Journal of biomechanics 2023-01-01 [PMID: 36682211] (IHC, Mouse)
• Perazza LR, Mitchell PL, Lizotte F et al. Fish oil replacement prevents, while docosahexaenoic acid-derived protectin DX mitigates end-stage-renal-disease in atherosclerotic diabetic mice FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021-05-01 [PMID: 33835594] (IF/IHC)
• Denhez B, Rousseau M, Spino C et al. Saturated fatty acids induce insulin resistance in podocytes through inhibition of IRS1 via activation of both IKK beta and mTORC1 Scientific reports 2020-12-10 [PMID: 33303821] (IHC-P, Mouse)
• Lizotte F, Denhez B, Guay A et al. Persistent Insulin Resistance in Podocytes Caused by Epigenetic Changes of SHP-1 in Diabetes. Diabetes. 2016-12-01 [PMID: 27585521]
• Qiao C, Meng F, Jang I et al. Deep transcriptomic profiling reveals the similarity between endothelial cells cultured under static and oscillatory shear stress conditions Physiol. Genomics 2016-07-22 [PMID: 27449656] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: COL4A1
• Entrez:
  • 12826(Mouse)
• Uniprot:
  • P02463(Mouse)