Collagen IV Antibody (MC4-HA)

Product Discontinued

Collagen IV Antibody (MC4-HA) Summary

• Immunogen: The antibody reacts with the Collagen IV antigen.
• Specificity: This monoclonal antibody reacts with native human collagen type IV, and with heat- denatured collagen IV form in ELISA and immunoblots. There is no cross-reactivity with human collagen I, II, III and V. It stains exclusively basement membranes on cryostat sections of human tissues such as kidney, liver and heart. The extent of expression of the basement membrane component type IV collagen may be correlated with the degree of differentiation of basement membrane producing neoplasms. Neoplastic cells of invasive carcinomas often lack a continuous basement membrane. In diagnostic histopathology antibodies to type IV collagen may help to distinguish between non-invasive (benign or in situ) and invasive lesions. Furthermore the antibody is important in the differential diagnosis of bullous lesions in dermatopathology. In in vitro studies with endothelial cells and smooth muscle cells the antibody is helpful in detection of production collagen type IV. This antibody reacts also with dog collagen IV on cryostate and paraffin sections.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: COL4A1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: For immunohistological staining of basement membranes on cryostat sections of human tissues. In combination with anti-collagen type I. It enables to discriminate between benign and malignant forms of soft tissue tumors (integrity of basement membranes is for immunohistochemistry on frozen sections fixation with acetone for 5 minutes at -20C is recommended. Staining of paraffin material generally renders less successful results. Such material should be fixed with neutral formalin and proteolytic pretreatment.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Ascites
• Preservative: No Preservative
• Purity: Unpurified

Alternate Names for Collagen IV Antibody (MC4-HA)

• BSVD;BSVD1;COL4A1s;Collagen alpha-1(IV) chain;PADMAL;RATOR
• Collagen IV

Background

Collagen IV is a major constituent of the basement membranes along with laminins, proteoglycans and enactins. It is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. It can form insoluble fibers with high tensile strength. Collagen IV is useful in detecting the loss of parts of basement membranes in carcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: COL4A1