Collagen IV alpha 1 Antibody (MC4-HA) Summary
The antibody reacts with the Collagen IV antigen.
This monoclonal antibody reacts with native human collagen type IV, and with heat- denatured collagen IV form in ELISA and immunoblots. There is no cross-reactivity with human collagen I, II, III and V. It stains exclusively basement membranes on cryostat sections of human tissues such as kidney, liver and heart. The extent of expression of the basement membrane component type IV collagen may be correlated with the degree of differentiation of basement membrane producing neoplasms. Neoplastic cells of invasive carcinomas often lack a continuous basement membrane. In diagnostic histopathology antibodies to type IV collagen may help to distinguish between non-invasive (benign or in situ) and invasive lesions. Furthermore the antibody is important in the differential diagnosis of bullous lesions in dermatopathology. In in vitro studies with endothelial cells and smooth muscle cells the antibody is helpful in detection of production collagen type IV. This antibody reacts also with dog collagen IV on cryostate and paraffin sections.
Test in a species/application not listed above to receive a full credit towards a future purchase.
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
This product is unpurified. The exact concentration of antibody is not quantifiable.
For immunohistological staining of basement membranes on cryostat sections of human tissues. In combination with anti-collagen type I. It enables to discriminate between benign and malignant forms of soft tissue tumors (integrity of basement membranes is for immunohistochemistry on frozen sections fixation with acetone for 5 min. at -20 degrees C is recommended. Staining of paraffin material generally renders less successful results. Such material should be fixed with neutral formalin and proteolytic pretreatment
Alternate Names for Collagen IV alpha 1 Antibody (MC4-HA)
- alpha-1 chain
- COL4A1 NC1 domain
- collagen alpha-1(IV) chain
- collagen IV, alpha-1 polypeptide
- collagen, type IV, alpha 1
- EC 3.4.23
- EC 188.8.131.52
Collagen IV is a major constituent of the basement membranes along with laminins, proteoglycans and enactins. It is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. It can form insoluble fibers with high tensile strength. Collagen IV is useful in detecting the loss of parts of basement membranes in carcinomas.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
Customers Who Viewed This Item Also Viewed...
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu, Mu, Rt, Bv, Ma
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
Applications: WB, IHC, IHC-P
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-P
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Ch, GP
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Applications: WB, Simple Western
Applications: WB, ELISA, ICC/IF, S-ELISA
Applications: ELISA, PLA, S-ELISA
Applications: WB, IHC
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC
Species: Hu, Pm, Rb
Applications: WB, ICC/IF
Applications: ELISA, IHC, IHC-Fr, IHC-P
Publications for Collagen IV alpha 1 Antibody (NBP1-42233) (0)
There are no publications for Collagen IV alpha 1 Antibody (NBP1-42233).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for Collagen IV alpha 1 Antibody (NBP1-42233) (0)
There are no reviews for Collagen IV alpha 1 Antibody (NBP1-42233).
By submitting a review earn points towards our Rewards Program.
- 250 points for product review
- 500 additional points for an image with your product review
- Double points (500) if you are the first to review this product
- Double points (1000) if you are the first to review this product with an image
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for Collagen IV alpha 1 Antibody (NBP1-42233) (0)
Additional Collagen IV alpha 1 Antibody (MC4-HA) Products
Related Products by Gene
Bioinformatics Tool for Collagen IV alpha 1 Antibody (NBP1-42233)
Discover related pathways, diseases and genes to Collagen IV alpha 1 Antibody (NBP1-42233). Need help?
Read the Bioinformatics Tool Guide
for instructions on using this tool.
Blogs on Collagen IV alpha 1.