Collagen IV alpha 1 Antibody (MC4-HA)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, IHC, IHC-Fr, IHC-P
Clone
MC4-HA
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Collagen IV alpha 1 Antibody (MC4-HA) Summary

Immunogen
The antibody reacts with the Collagen IV antigen.
Specificity
This monoclonal antibody reacts with native human collagen type IV, and with heat- denatured collagen IV form in ELISA and immunoblots. There is no cross-reactivity with human collagen I, II, III and V. It stains exclusively basement membranes on cryostat sections of human tissues such as kidney, liver and heart. The extent of expression of the basement membrane component type IV collagen may be correlated with the degree of differentiation of basement membrane producing neoplasms. Neoplastic cells of invasive carcinomas often lack a continuous basement membrane. In diagnostic histopathology antibodies to type IV collagen may help to distinguish between non-invasive (benign or in situ) and invasive lesions. Furthermore the antibody is important in the differential diagnosis of bullous lesions in dermatopathology. In in vitro studies with endothelial cells and smooth muscle cells the antibody is helpful in detection of production collagen type IV. This antibody reacts also with dog collagen IV on cryostate and paraffin sections.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
COL4A1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
For immunohistological staining of basement membranes on cryostat sections of human tissues. In combination with anti-collagen type I. It enables to discriminate between benign and malignant forms of soft tissue tumors (integrity of basement membranes is for immunohistochemistry on frozen sections fixation with acetone for 5 min. at -20 degrees C is recommended. Staining of paraffin material generally renders less successful results. Such material should be fixed with neutral formalin and proteolytic pretreatment

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Ascites
Preservative
No Preservative
Purity
Unpurified

Alternate Names for Collagen IV alpha 1 Antibody (MC4-HA)

  • alpha-1 chain
  • arresten
  • COL4A1 NC1 domain
  • COL4A1
  • collagen alpha-1(IV) chain
  • Collagen IV alpha 1
  • collagen IV, alpha-1 polypeptide
  • collagen, type IV, alpha 1
  • Collagen-4
  • EC 3.4.23
  • EC 6.3.1.2

Background

Collagen IV is a major constituent of the basement membranes along with laminins, proteoglycans and enactins. It is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. It can form insoluble fibers with high tensile strength. Collagen IV is useful in detecting the loss of parts of basement membranes in carcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol COL4A1
Entrez