COL4A3BP Antibody

Western Blot: COL4A3BP Antibody [NBP1-06028] (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

COL4A3BP Antibody Summary

• Immunogen: Peptide with sequence C-KREDSWQKRLDKETEK corresponding to internal region according to NP_005704.1, NP_112729.1.
• Epitope: C-KREDSWQKRLDKETEK
• Specificity: This antibody is expected to recognize both reported isoforms (NP_005704.1; NP_112729.1).
• Predicted Species: Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CERT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 70 kDa band observed in human heart lysates (calculated MW of 70.8 kDa band according to NP_005704.1).
• Control:

  COL4A3BP Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for COL4A3BP Antibody

• Ceramide transfer protein
• CERTFLJ20597
• CERTL
• collagen type IV alpha-3-binding protein
• collagen, type IV, alpha 3 (Goodpasture antigen) binding protein
• Goodpasture antigen-binding protein
• GPBPSTARD11ceramide transporter
• hCERT
• lipid-transfer protein CERTL
• StARD11
• StAR-related lipid transfer (START) domain containing 11
• StAR-related lipid transfer protein 11
• START domain containing 11
• START domain-containing protein 11

Background

This gene encodes a kinase that specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. Goodpasture disease is the result of an autoimmune response directed at this antigen. One isoform of this protein is also involved in ceramide intracellular transport. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for COL4A3BP Antibody (NBP1-06028)(1)

Publication using NBP1-06028

• Kudo N, Kumagai K, Tomishige N et al. Structural basis for specific lipid recognition by CERT responsible for nonvesicular trafficking of ceramide. Proc Natl Acad Sci U S A 2008-01-15 [PMID: 18184806]

Bioinformatics

• Gene Symbol: CERT1
• Entrez:
  • 10087(Human)
• Uniprot:
  • NP_005704.1(Human)