COL4A3BP Antibody

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Western Blot: COL4A3BP Antibody [NB100-93386] - (0.5ug/ml) staining of mouse heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

COL4A3BP Antibody Summary

Immunogen
Peptide with sequence DHDSAPLNNRC corresponding to internal region according to NP_005704.1, NP_112729.1.
Epitope
DHDSAPLNNRC
Specificity
This antibody is expected to recognise both reported isoforms (NP_005704.1 and NP_112729.1)
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
COL4A3BP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1.5 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 60 kDa band observed in mouse heart lysates (calculated MW of 71.1 kDa band according to mouse NP_075909.1 and 60.0 kDa band according to human NP_112729.1). An additional band of unknown identity was also consistently observed at 26 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Positive Control
COL4A3BP Lysate (NBL1-09354)
Publications
Read Publication using NB100-93386.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Human.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for COL4A3BP Antibody

  • Ceramide transfer protein
  • CERTFLJ20597
  • CERTL
  • collagen type IV alpha-3-binding protein
  • collagen, type IV, alpha 3 (Goodpasture antigen) binding protein
  • Goodpasture antigen-binding protein
  • GPBPSTARD11ceramide transporter
  • hCERT
  • lipid-transfer protein CERTL
  • StARD11
  • StAR-related lipid transfer (START) domain containing 11
  • StAR-related lipid transfer protein 11
  • START domain containing 11
  • START domain-containing protein 11

Background

This gene encodes a kinase that specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. Goodpasture disease is the result of an autoimmune response directed at this antigen. One isoform of this protein is also involved in ceramide intracellular transport. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for COL4A3BP Antibody (NB100-93386)(1)

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol COL4A3BP
Entrez
Uniprot