Coagulation Factor II/Thrombin Antibody [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Coagulation Factor II/Thrombin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1148) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Coagulation Factor II/Thrombin at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Western blot shows human plasma. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Coagulation Factor II/Thrombin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1148) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Coagulation Factor II/Thrombin at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

HepG2 human hepatocellular carcinoma cell line was stained with Goat Anti-Human Factor II/Thrombin Polyclonal Antibody (Catalog # AF1148, filled histogram) or control antibody (AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Human peripheral blood monocytes were stained with Goat Anti-Human Coagulation Factor II/Thrombin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1148, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Coagulation Factor II/Thrombin at approximately 95 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Coagulation Factor II/Thrombin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1148) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Coagulation Factor II/Thrombin Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor II/Thrombin
• Specificity: Detects human Coagulation Factor II/Thrombin in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) PROC is observed, and less than 1% cross-reactivity with rhFactor X, recombinant mouse (rm) Factor X and rmPROC is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: F2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Simple Western 10 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Coagulation Factor II/Thrombin Antibody [Unconjugated]

• coagulation factor II (thrombin) receptor-like 2
• Coagulation factor II receptor-like 2 (protease-actovated receptor 3)
• Coagulation factor II receptor-like 2
• Coagulation Factor II
• F2
• PAR-3
• PAR3proteinase-activated receptor 3
• protease-activated receptor 3
• proteinase-activated receptor-3
• PT
• serine protease
• Thrombin receptor-like 2
• Thrombin

Background

Coagulation Factor II, commonly known as thrombin, is an essential component of the coagulation cascade in which it converts fibrinogen to fibrin, activates factors V, VII, VIII, XIII and forms complexes with protein C and thrombomodulin (1). It also activates platelets and regulates the behavior of additional cells through protease-activated receptors (PARs) (2). It may have either protective or deleterious functions, depending on the level and location (3). Its activity is regulated by endogenous inhibitors such as anti-thrombin III (serpin C1) or heparin cofactor II (serpin D1). A plasma serine protease, thrombin is synthesized in the liver as a 622 amino acid precursor with a 24 amino acid signal peptide. Cleavage by itself or by similar enzymes converts the proenzyme to three forms designated as alpha -, beta - and gamma -thrombin. Composed of a disulfide bond-linked dimer of the light chain (A) (residues 328-363) and the heavy chain (B) (residues 364-622), alpha -thrombin displays the diverse functions as described above. In comparison, the further processed B chains of beta - and gamma -thrombin have no known physiological function, but retain most of the activity towards small synthetic substrates (4).

1. Degen, S.J. and E.W. Davie (1987) Biochemistry 26:6165.
2. Coughlin, S.R. (2000) Nature 407:258.
3. Xi, G. et al. (2003) J. Neurochem. 84:3.
4. Rydel, T.J. et al. (1994) J. Biol. Chem. 269:22000.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Coagulation Factor II/Thrombin Antibody (AF1148)(1)

Publication using AF1148

• Jesmin S, Gando S, Zaedi S, Prodhan SH, Sawamura A, Miyauchi T, Hiroe M, Yamaguchi N Protease-activated receptor 2 blocking peptide counteracts endotoxin-induced inflammation and coagulation and ameliorates renal fibrin deposition in a rat model of acute renal failure. Shock, 2009-12-01;32(6):626-32. 2009-12-01 [PMID: 19333145] (Western Blot, Rat)

FAQs for Coagulation Factor II/Thrombin Antibody (AF1148). (Showing 1 - 1 of 1 FAQ).

1. Do you have neutralizing antibodies for anti-mouse Thrombin?
   • We currently have 1 neutralizing Thrombin antibody (NBP1-95029), however it is an anti-human antibody and has only been validated in human samples. If you would like to test this antibody in mouse samples, you may be interested in participating in our Innovators Reward Program.

Bioinformatics

• Gene Symbol: F2