CNPase Antibody

Images

 
Immunohistochemistry: CNPase Antibody [NB100-1935] - A tissue section through an adult mouse brain showing CNPase (brown staining) in white matter tracts and the granule cell layer of the cerebellum.
CNPase Antibody [NB100-1935] - CNPase-positive oligodendrocytes (brown) in cerebellar white matter (adult mouse). Right: Western.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

CNPase Antibody Summary

Immunogen
Chickens were immunized with a synthetic peptide/keyhole limpet hemocyanin (KLH) conjugate. The peptide corresponded to a region of CNPase shared between the human (NP_149124, NCBI) and mouse (P16330, NCBI) sequences.
Localization
Oligodendrocyte
Marker
Oligodendrocyte Marker
Isotype
IgY
Clonality
Polyclonal
Host
Chicken
Gene
CNP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:500-1:1000
  • Western Blot 1:500-1:1000
Application Notes
Quality assurance analysis was performed using immunohistochemistry 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500) as the secondary reagent. For immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells, a dilution of 1:1000-1:2000 was used. For western blots, a dilution of 1:2000-1:5000 was used.
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
10mM Sodium PBS (0.9% isotonic, w/v, pH 7.2)
Preservative
0.02% Sodium Azide
Concentration
0.1 mg/ml
Purity
Immunogen affinity purified

Notes

Chicken products cannot be exported to Canada.

Purification Notes
After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity-purified using a peptide column. The concentrations of the eluates were then adjusted to 100 ug/ml, and the preparation was filter-sterilized.

Storage Notes
Store at 4C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

Alternate Names for CNPase Antibody

  • 2', 3' cyclic nucleotide 3' phosphohydrolase
  • 2'-3'-cyclic nucleotide 3' phosphodiesterase
  • 2'-3'-cyclic-nucleotide 3'-phosphodiesterase
  • CNP1
  • CNPase
  • EC 3.1.4.37

Background

2,3-cyclic nucleotide-3-phosphodiesterase (CNP) is a membrane bound, microtubule associated protein that is among the most abundant myelin proteins of the CNS. It is thought that CNP may serve as a regulator of tubulin polymerization and of microtubule distribution (Bifulco et al., 2002). It was recently found that CNP may also function as a possible linker protein anchoring microtubules to the plasma membrane via a 13 residue C-terminal CNP fragment (Bifulco et al., 2002, Esposito et al., 2008).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CNPase Antibody (NB100-1935). (Showing 1 - 1 of 1 FAQ).

  1. According to datasheet, this product contains sodium azide, so please tell the exact concentration. Because in Japan sodium azide is toxic over 0.1%.
    • The CNPase Antibody (NB100-1935) contains 0.25ml of peptide affinity purified chicken antisera in PBS, pH 7.2 with 0.02% sodium azide preservative.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CNP
Entrez
Uniprot