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CNOT7 Antibody

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Western Blot: CNOT7 Antibody [NBP2-92571] - Analysis of extracts of various cell lines, using CNOT7 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Western Blot: CNOT7 Antibody [NBP2-92571] - Analysis of extracts of various cell lines, using CNOT7 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CNOT7 Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 90-160 of human CNOT7 (NP_037486.2).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CNOT7
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:200-1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for CNOT7 Antibody

  • BTG1 binding factor 1
  • BTG1-binding factor 1
  • CAF-1
  • CAF1carbon catabolite repressor protein (CCR4)-associative factor 1
  • CCR4-associated factor 1
  • CCR4-NOT transcription complex subunit 7
  • CCR4-NOT transcription complex, subunit 7
  • hCAF-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-15930
Western Blot: CNOT8 Antibody [NBP2-15930] - Sample (30 ug of whole cell lysate) A: SK-N-SH 12% SDS PAGE gel, diluted at 1:500.
Immunocytochemistry/Immunofluorescence: CNOT8 Antibody [NBP2-15930] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CNOT8 protein stained by CNOT8 antibody diluted at 1:200. Blue: Hoechst 33342 staining.
Species: Hu
Applications: ICC/IF, WB
CNOT6 Antibody
NBP1-86564
Immunohistochemistry-Paraffin: CNOT6 Antibody [NBP1-86564] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunocytochemistry/Immunofluorescence: CNOT6 Antibody [NBP1-86564] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Nocturnin Antibody
NBP2-37856
Immunohistochemistry-Paraffin: Nocturnin Antibody [NBP2-37856] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunocytochemistry/Immunofluorescence: Nocturnin Antibody [NBP2-37856] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
CCR4 Antibody
(205410) [Unconjugate
MAB1567
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse Anti-Human CCR4 Monoclonal Antibody (Catalog # MAB1567) or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
(19 Publications)
Species: Hu
Applications: CyTOF-reported, Flow
POPDC2 Antibody
NBP1-87256
Immunohistochemistry-Paraffin: POPDC2 Antibody [NBP1-87256] - Analysis in human heart muscle and skeletal muscle tissues. Corresponding POPDC2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: POPDC2 Antibody [NBP1-87256] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: POPDC2 Antibody [NBP1-87256] - Staining of human heart muscle shows moderate membranous positivity in cardiomyocytes.
Species: Hu
Applications: IHC, IHC-P
CHAF1A Antibody
(SS 1 1-13)
NB500-207
Western Blot: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Analysis of CAF1 p150 expression in Ntera2 whole cell lysate using NB500-207.
Immunocytochemistry/Immunofluorescence: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Immunofluorescent staining of CAF-1-p150 in HeLa cells (Shibahara et al 1999).
(10 Publications)
Species: Hu
Applications: ICC/IF, IP, WB
CHAF1B Antibody
(SS 53)
NB500-212
Knockdown Validated: CHAF1B Antibody (SS 53) [NB500-212] - A. RKO cells with CAF-1 p150, CAF-1 p60 and PCNA antibodies. B. p150 RNAi knockdown. Reference Pubmed 14519857.
(8 Publications)
Species: Hu
Applications: ICC/IF, IP, KD, WB
PCNA Antibody
(PC10)
NB500-106
Immunocytochemistry/Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - HeLa cells were fixed and permeabilized for 10 minutes using cold (-20C) MeOH. The cells were incubated with anti-PCNA [PC10] at 5 ug/mL overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: PCNA Antibody (PC10) [NB500-106] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Flow Cytometry: PCNA Antibody (PC10) [NB500-106] - An intracellular stain was performed on HepG2 cells with PCNA Antibody (PC10) NB500-106G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.
(5 Reviews)
(37 Publications)
Species: Ch, Dr, Fi, Hu, Mar, Mu, Po, Pm, Rb, Rt, Ye, Ze
Applications: ChIP, ELISA, Flow, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
RBBP4/RbAp48
Antibody (11G10)
NB500-123
Immunoprecipitation: RBBP4/RbAp48 Antibody (11G10) [NB500-123] - Myc-tag antibody immunoprecipitation confirmed pulldown of NuRD component RbAp48 which is known to interact with ZNF827. * denotes IgG band at 55 kDa. Image submitted by a verified customer review.
Immunohistochemistry: RbAp48 Antibody (1D11) [NB500-123]
Immunocytochemistry/Immunofluorescence: RbAp48 Antibody (1D11) [NB500-123] - Immunofluorescence analysis of HeLa, using RbAp48 antibody at 1:100 dilution.
(1 Review)
(5 Publications)
Species: Hu, Mu
Applications: B/N, ChIP, Func, ICC/IF, IHC, IHC-P, IP, WB
HIST1H4F
Overexpression Lysate
NBP2-07996
Western Blot: HIST1H4F Overexpression Lysate (Adult Normal) [NBP2-07996] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIST1H4F.
Species: Hu
Applications: WB
H4/n Antibody
(3E7H2) - BSA Free
NBP2-52486
ELISA: H4/n Antibody (3E7H2) [NBP2-52486] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).
Western Blot: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis using HIST2H4A(20Me) mouse mAb against THP-1 (1) cell lysate.
Flow Cytometry: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis of Raji cells using HIST2H4A(20Me) mouse mAb (green) and negative control (red).
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB
Histone H3
[Trimethyl Lys9] Anti ...
NBP1-30141
Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Observed molecular weight is ~15 kDa.
Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Representative Western blot showing M-track protein protein proximity signals obtained for Kic1. Hog1-protA-H3: background control, Nup2: positive control. Below: Proximity signals. n = 3 replicates per sample except when indicated differently. Ratios are log2-transformed. Black lines indicate average proximity signal. Proximity signals that differ significantly from background are marked in green (q 0.01) filled circles. Grey filled triangles: q > 0.05. N: N-terminal HKMTmyc fusion. Image collected and cropped by CiteAb from the following publication (https://biosignaling.biomedcentral.com/articles/10.1186/s12964-019-0381-z), licensed under a CC-BY licence.
Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Analysis of Histone H3 K9-me3 in HeLa histone lysates. Observed molecular weight is ~15 kDa.
(2 Reviews)
(23 Publications)
Species: Ce, Dr, Hu, In, Ma, Mu, Po, Rt, Ye
Applications: ChIP, ELISA, ICC/IF, IHC, IHC-P, IP, WB
TOB1 Antibody
AF5228
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Human TOB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5228) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TOB1 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Species: Hu
Applications: WB
BTG2 Antibody
(OTI4C10)
NBP2-46587
Immunohistochemistry: BTG1 Antibody (4C10) [NBP2-46587] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: BTG1 Antibody (4C10) [NBP2-46587] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: BTG1 Antibody (4C10) [NBP2-46587] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Species: Hu, Mu, Rt
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CNOT1 Antibody
NBP2-31892
Immunohistochemistry-Paraffin: CNOT1 Antibody [NBP2-31892] - Staining of human fallopian tube shows weak to moderate cytoplasmic and membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: CNOT1 Antibody [NBP2-31892] - Staining of human duodenum shows moderate to strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: CNOT1 Antibody [NBP2-31892] - Staining of human placenta shows moderate to strong granular cytoplasmic positivity in trophoblastic cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
TNF RI/TNFRSF1A
[HRP]
DRT100
(2 Reviews)
(76 Publications)
Species: Hu
Applications: ELISA
Histamine H4R
Antibody - BSA Free
NLS3775
Immunohistochemistry-Paraffin: Histamine H4R Antibody [NLS3775] - Ovary, carcinoma
Immunohistochemistry-Paraffin: Histamine H4R Antibody [NLS3775] - Pancreas, carcinoma
Immunohistochemistry-Paraffin: Histamine H4R Antibody [NLS3775] - Colon, Carcinoma
(1 Publication)
Species: Hu, Pm
Applications: ICC/IF, IHC, IHC-P
CCDC6 Antibody
NBP1-85351
Immunohistochemistry-Paraffin: CCDC6 Antibody [NBP1-85351] - Staining of human fallopian tube shows high expression.
Immunohistochemistry-Paraffin: CCDC6 Antibody [NBP1-85351] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: CCDC6 Antibody [NBP1-85351] - Staining in human fallopian tube and skeletal muscle tissues using anti-CCDC6 antibody. Corresponding CCDC6 RNA-seq data are presented for the same tissues.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
BTG1 Antibody
(OTI7H6)
NBP2-46586
Immunohistochemistry: BTG1 Antibody (7H6) [NBP2-46586] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: BTG1 Antibody (7H6) [NBP2-46586] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: BTG1 Antibody (7H6) [NBP2-46586] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P
Fas/TNFRSF6/CD95
[HRP]
DFS00
(1 Review)
(17 Publications)
Species: Hu
Applications: ELISA

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Bioinformatics

Gene Symbol CNOT7