CMIP Antibody - Azide and BSA Free

Western Blot: CMIP Antibody [NBP3-16024] - Western blot analysis of extracts of various cell lines, using CMIP antibody (NBP3-16024) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Western Blot: CMIP Antibody [NBP3-16024] - Western blot analysis of extracts of K-562 cells, using CMIP antibody (NBP3-16024) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Immunohistochemistry-Paraffin: CMIP Antibody [NBP3-16024] - Immunohistochemistry of paraffin-embedded mouse fetal liver using CMIP Rabbit pAb (NBP3-16024) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CMIP Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit CMIP Antibody - Azide and BSA Free (NBP3-16024) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 640-773 of human CMIP (NP_938204.2). RLTLPSKSTDADLARLLSSGSFGNLENLSLAFTNVTSACAEHLIKLPSLKQLNLWSTQFGDAGLRLLSEHLTMLQVLNLCETPVTDAGLLALSSMKSLCSLNMNSTKLSADTYEDLKAKLPNLKEVDVRYTEAW
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CMIP
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 86 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for CMIP Antibody - Azide and BSA Free

• c-Mip
• KIAA1694c-Maf-inducing protein
• T
• TCMIP
• tc-Mip
• Truncated c-Maf-inducing protein

Background

CMIP plays a role in T-cell signaling pathway. Isoform 2 may play a role in T-helper 2 (Th2) signaling pathway andseems to represent the first proximal signaling protein that links T-cell receptor-mediated signal to the activationof c-Maf Th2 specific factor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CMIP