CLEC9a Antibody

CLEC9a was detected in perfusion fixed frozen sections of mouse spleen using Sheep Anti-Mouse CLEC9a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6776) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to dendritic cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

CLEC9a Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse CLEC9a
  Lys57-Ile264
  Accession # EDK99924
• Specificity: Detects mouse CLEC9a in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CLEC9a, rhCLEC2, and rhCLEC12B is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CLEC9A
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CLEC9a Antibody

• CD370
• CLEC9a
• C-type lectin domain family 9 member A
• C-type lectin domain family 9, member A
• DNGR1
• HEEE9341
• UNQ9341

Background

CLEC9a (C-type lectin domain family member A; also DNGR-1) is a 50-52 kDa member of the group V C-type lectin domain containing family of receptors. In mouse, it is an activation receptor expressed on both CD8 alpha + CD24+ and plasmacytoid dendritic cells (DC). It assists in endocytosis but not phagocytosis, and appears to induce cytotoxic antitumor T cells by participating in class I MHC antigen presentation. CD8 alpha + DCs are believed to be particularly important for the disposal of dead cell material. Mouse CLEC9a is a 264 amino acid (aa) type II transmembrane glycoprotein. It contains a 35 aa cytoplasmic segment (aa 1-35) and a 208 aa extracellular region (aa 57-264) that possesses one C-type lectin domain (aa 144-256). CLEC9a forms disulfide-linked homodimers on the cell surface. There are multiple splice variants. The CLEC9a SwissProt entry (Q8BRU4) is 238 aa in length and shows a deletion of aa 106-131. Other isoforms contain either a 43 or a 67 aa substitution for aa 106-264, a Val substitution for 31-58, and a combination of the just mentioned Val substitution coupled to a deletion of aa 106-131. Both rat and human CLEC9a appear to be absent aa 106-131 found in full-length mouse CLEC9a. Taking this into account, over aa 57-264, mouse CLEC9a shares 50% and 70% aa identity with human and rat CLEC9a, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CLEC9a Antibody (AF6776)(3)

Publications using AF6776

• Ana-Sosa-Batiz, F;Verma, SK;Shafee, N;Miller, R;Conner, C;Hastie, KM;Timis, J;Maule, E;Nguyen, MN;Tran, L;Varghese, K;Madany, H;Street, AE;Zandonatti, M;Moi, ML;Jarnagin, K;Webb, DR;Saphire, EO;Kim, K;Shresta, S; A humanised ACE2, TMPRSS2, and FCGRT mouse model reveals the protective efficacy of anti-receptor binding domain antibodies elicited by SARS-CoV-2 hybrid immunity EBioMedicine 2025-02-27 [PMID: 40020261] (Western Blot, Mouse)
• C Pleuger, D Ai, ML Hoppe, LT Winter, D Bohnert, D Karl, S Guenther, S Epelman, C Kantores, M Fijak, S Ravens, R Middendorf, JU Mayer, KL Loveland, M Hedger, S Bhushan, A Meinhardt The regional distribution of resident immune cells shapes distinct immunological environments along the murine epididymis Elife, 2022-12-14;11(0):. 2022-12-14 [PMID: 36515584] (IHC, Mouse)
• M Valente, Y Dölen, E van Dinthe, L Vimeux, M Fallet, V Feuillet, CG Figdor Cross-talk between iNKT cells and CD8 T cells in the spleen requires the IL-4/CCL17 axis for the generation of short-lived effector cells Proc. Natl. Acad. Sci. U.S.A., 2019-12-03;0(0):. 2019-12-03 [PMID: 31796596] (Confocal Microscopy, Mouse)

Bioinformatics

• Gene Symbol: CLEC9A
• Uniprot:
  • EDK99924()