CLEC9a Antibody (7H11)

Mouse splenocytes were stained with Anti-Mouse Integrin alpha X/CD11c PE-conjugated Monoclonal Antibody and either (A) Rat Anti-Mouse CLEC9a Monoclonal Antibody (Catalog # MAB67761) or (B) Rat IgG1 Isotype Control (Catalog # MAB005) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready, ICC/IF
• Clone: 7H11
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

CLEC9a Antibody (7H11) Summary

• Immunogen: RBL‑2H3 rat basophilic leukemia cell line expressing mouse CLEC9a fused to an HA epitope
  Accession # Q8BRU4
• Specificity: Detects mouse CLEC9a.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: CLEC9A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 uL/10^6 cells
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CLEC9a Antibody (7H11)

• CD370
• CLEC9a
• C-type lectin domain family 9 member A
• C-type lectin domain family 9, member A
• DNGR1
• HEEE9341
• UNQ9341

Background

CLEC9a (C-type lectin domain family 9 member A), also known as DNGR-1, is a type II transmembrane glycoprotein member of the C-type lectin superfamily. Although the CTLD of CLEC9a structurally resembles that of other C-type lectins, it lacks the conserved residues that typically mediate calcium and carbohydrate binding. CLEC9a is expressed as a disulfide-linked homodimer of approximately 50 kDa N-glycosylated subunits. Human CLEC9a expression is restricted to a subpopulation of BDCA-3⁺ conventional dendritic cells (cDC) and CD16- monocytes. BDCA-3⁺ cDC are analagous to mouse CD8⁺ cDC which are specialized in antigenic cross-presentation in complex with MHC class I molecules. In mouse, CLEC9a is additionally expressed on plasmacytoid dendritic cells. CLEC9a ligation enhances antigen uptake and processing, leading to presentation on MHC class I and cytotoxic T cell (CTL) priming. In mouse, CLEC9a recognizes normally intracellular determinant(s) of necrotic cells and mediates their uptake by the dendritic cell. The subsequent antigenic cross-presentation to CTL is important for clearing necrotic cellular debris. CLEC9a signaling triggers activation of the tyrosine kinase Syk. Alternative splicing of mouse CLEC9a generates isoforms with deletions in the transmembrane segment, stalk region, or CTLD. Within aa 57-264 of the ECD, mouse CLEC9a shares 57% and 80% aa sequence identity with human and rat CLEC9a, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CLEC9a Antibody (MAB67761)(4)

Publications using MAB67761

• Li J, Ahmet F, Sullivan L, Brooks A, Kent S, De Rose R, Salazar A, Reis E Sousa C, Shortman K, Lahoud M, Heath W, Caminschi I Antibodies targeting Clec9A promote strong humoral immunity without adjuvant in mice and non-human primates. Eur J Immunol, 2015-01-14;45(3):854-64. 2015-01-14 [PMID: 25487143] (Neutralization, Mouse)
• Picco G, Beatson R, Taylor-Papadimitriou J, Burchell J Targeting DNGR-1 (CLEC9A) with antibody/MUC1 peptide conjugates as a vaccine for carcinomas. Eur J Immunol, 2014-04-17;44(7):1947-55. 2014-04-17 [PMID: 24648154] (Functional Assay, Mouse)
• Caminschi I, Shortman K Boosting antibody responses by targeting antigens to dendritic cells. Trends Immunol, 2011-12-06;33(2):71-7. 2011-12-06 [PMID: 22153931] (Functional Assay)
• Joffre O, Sancho D, Zelenay S, Keller A, Reis E Sousa C Efficient and versatile manipulation of the peripheral CD4+ T-cell compartment by antigen targeting to DNGR-1/CLEC9A. Eur J Immunol, 2010-05-01;40(5):1255-65. 2010-05-01 [PMID: 20333625] (ELISA Development (Capture), Mouse)

Bioinformatics

• Gene Symbol: CLEC9A
• Uniprot:
  • Q8BRU4(Mouse)