CLEC3B/Tetranectin Antibody (JG37-16)

Western Blot: CLEC3B/Tetranectin Antibody (JG37-16) [NBP2-75442] - Analysis of Tetranectin on rat skin (1) and human skin (2) tissue lysates using anti-Tetranectin antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: CLEC3B/Tetranectin Antibody (JG37-16) [NBP2-75442] - Staining Tetranectin in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CLEC3B/Tetranectin Antibody (JG37-16) [NBP2-75442] - Analysis of paraffin-embedded human kidney tissue using anti-Tetranectin antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: CLEC3B/Tetranectin Antibody (JG37-16) [NBP2-75442] - Staining Tetranectin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CLEC3B/Tetranectin Antibody (JG37-16) [NBP2-75442] - Analysis of paraffin-embedded human liver cancer tissue using anti-Tetranectin antibody. Counter stained with hematoxylin.

Product Discontinued

CLEC3B/Tetranectin Antibody (JG37-16) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human CLEC3B/Tetranectin aa 110-202 / 202. (SwissProt: P05452 Human; SwissProt: P43025 Mouse)
• Localization: Secreted.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CLEC3B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:1000-1:5000
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CLEC3B/Tetranectin Antibody (JG37-16)

• CLEC3B
• C-type lectin domain family 3 member B
• C-type lectin domain family 3, member B
• DKFZp686H17246
• Plasminogen kringle 4-binding protein
• TETN
• tetranectin (plasminogen binding protein)
• Tetranectin
• TNA
• TNAtetranectin (plasminogen-binding protein)
• TNtetranectin

Background

Tetranectin (TN) is a serum and tissue protein, a C-type lectin, which binds to Ca++. It is a homotrimer of monomers each with a mass of 20 kDa, plasma or serum concentrations of TN are found to be approximately 10 mg/l (1,2,4). In vitro, TN can bind to kringle 4 of plasminogen and enhance the activation of plaminogen to plasmin, catalyzed by tissue plasminogen activator in the presence of poly-D-lysine (3). TN is best known as a prognostic marker in ovarian cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLEC3B