CLEC17A Antibody (1064719) [Allophycocyanin]

HEK293 cells transfected with Human CLEC17A and eGFP were stained with either (A) Mouse Anti-Human CLEC17A APC‑conjugated Monoclonal Antibody (Catalog # FAB101471A) or (B) Mouse IgG1 Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 1064719
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

CLEC17A Antibody (1064719) [Allophycocyanin] Summary

• Immunogen: CHO-derived human CLEC17A
  Lys194-Cys378
  Accession # Q6ZS10
• Specificity: Detects human CLEC17A in direct ELISA.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CLEC17A Antibody (1064719) [Allophycocyanin]

• CLEC17A
• C-type lectin domain family 17, member A
• Prolectin

Background

C-type lectin domain family 17, member A (CLEC17A), also known as Prolectin, is type II transmembrane protein that is expressed mainly on dividing B cells found in the germinal centers of secondary lymphoid organs, including lymph nodes, tonsils, stomach, intestine, appendix and spleen (1, 2). CLEC17A binds preferentially to epithelial rather than to mesenchymal cells, and it behaves as a cell adhesion molecule for epithelial cells (2).  It has high specificity towards mannose and was found to form disulfide-linked oligomers (1, 3). Human CLEC17A is synthesized as a 378 amino acid (aa) protein that includes a 172 aa cytoplasmic domain, a 21 aa transmembrane segment, and a 185 aa extracellular domain (ECD). Within the ECD, human CLEC17A shares 84% aa sequence identity with canine CLEC17A. C-type lectins are Ca²⁺-depending sugar-binding proteins that are involved in several immune-related and other physiological functions. Presently, 17 groups within the C-type lectin superfamily have been recognized (4), with more C-type lectins being constantly discovered based on the presence of a conserved 115-130 amino acid domain along their sequences - the C-type carbohydrate recognition domain (CRD). However, for most of the recently identified C-type lectins, their interactions with carbohydrates, intracellular functions and molecular mechanisms still remain unclear (3).

1. Graham, S. A. et al. (2009) J. Biol. Chem. 284:18537.
2. Breiman, A. et al. (2016) Oncotarget 7:14064.
3. Koh, G. et al. (2011) BMC Bioinformatics 12:S5.
4. Zelensky AN. et al. (2005) FEBS Journal 272:6179.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q6ZS10()