CLEC-2/CLEC-1B Antibody (219133) [Allophycocyanin]

Human whole blood was stained with Mouse Anti-Human CD41 FITC-conjugated Monoclonal Antibody and either (A) Mouse Anti-Human CLEC-2 APC-conjugated Monoclonal Antibody (Catalog # FAB1718A) or (B) Mouse IgG2AAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Platelets were stained with Mouse Anti-Human Integrin alpha 2b/CD41 PE‑conjugated Monoclonal Antibody (Catalog # FAB7616P) and either (A) Mouse Anti-Human CLEC-2/CLEC1B APC‑conjugated Monoclonal Antibody (Catalog # FAB1718A) or (B) Mouse IgG2B Allophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 219133
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

CLEC-2/CLEC-1B Antibody (219133) [Allophycocyanin] Summary

• Immunogen: NS0-derived rhCLEC-2 extracellular domain
  Gln58-Pro229
  Accession # AAF36777
• Specificity: Detects human CLEC-2 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant human CLEC‑1.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLEC1B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CLEC-2/CLEC-1B Antibody (219133) [Allophycocyanin]

• 1810061I13Rik
• CLEC1B
• CLEC2
• CLEC-2
• CLEC2B
• CLEC2PRO1384
• C-type lectin domain family 1 member B
• C-type lectin domain family 1, member B
• C-type lectin-like receptor 2
• C-type lectin-like receptor-2
• QDED721

Background

C-type lectin-like receptor 2 (CLEC-2) is a 32 kDa type II transmembrane glycoprotein and member of the C-type lectin-like family of receptors (1-4). CLEC-2 consists of a 33 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane region, and a 175 aa extracellular domain. The cytoplasmic domain contains multiple threonine and serine residues which are sites of potential phosphorylation, and a YXXL (Tyr-Xaa-Xaa-Leu) motif through which CLEC-2 does its signaling (2, 4-5). Ligand binding and cross-linking of CLEC-2 induces Src kinase-dependent tyrosine phosphorylation of the YXXL sequence, inducing activation of the tyrosine kinase Syk and initiation of a signaling pathway that culminates in activation of phospholipase C gamma 2 (2, 5). The extracellular domain contains three potential sites of N-linked glycosylation, and a single carbohydrate recognition domain (CRD) which shows conservation of six cysteine residues (1, 6). Unlike most other members of the C‑type lectin-like family of receptors, CLEC-2's CRD lacks the amino acid residues that are crucial for Ca²⁺-dependent carbohydrate binding, making it a non‑classical C-type lectin receptor (1, 6). A splicing variant at aa 22-55 produces two isoforms for CLEC-2. Isoform 1 is the longer protein, and in isoform 2, an alanine residue is substituted for aa 22-55. Human CLEC-2 shares 63% aa sequence identity with mouse CLEC-2. CLEC-2 is expressed preferentially in liver, and is also detected in myeloid cells (monocytes, dendritic cells, and granulocytes) (1), platelets, and megakaryocytes (4). CLEC-2 is the receptor for the platelet-aggregating snake venom protein rhodocytin (3-4) and the molecule podoplanin, a transmembrane sialoglycoprotein that, when bound to CLEC-2, is involved in platelet aggregation, tumor metastasis, and lymphatic vessel formation (2, 7). CLEC-2 has also been shown to enhance infectivity of HIV-1 by mediating HIV-1 attachment and transfer by CLEC-2 transfected cells and platelets (8).

1. Colonna, M. et al. (2000) Eur. J. Immunol. 30:697.
2. Christou, C.M. et al. (2008) Biochem. J. 411:133.
3. Watson, A.A. et al. (2007) J. Biol. Chem. 282:3165.
4. Suzuki-Inoue, K. et al. (2006) Blood 107:542.
5. Fuller, G.L. et al. (2007) J. Biol. Chem. 282:12397.
6. Weis, W.I. et al. (1998) Immunol. Rev. 163:19.
7. Suzuki-Inoue, K. et al. (2007) J. Biol. Chem. 282:25993.
8. Chaipan, C. et al. (2006) J. Virol. 80:8951.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CLEC-2/CLEC-1B Antibody (FAB1718A)(1)

Publication using FAB1718A

• Manisha Yadav, Md. Naushad Akhtar, Manish Mishra, Sandeep Kumar, Raj Kumar, Shubham et al. Leishmania donovani Attenuates Dendritic Cell Trafficking to Lymph Nodes by Inhibiting C-Type Lectin Receptor 2 Expression via Transforming Growth Factor-beta Microbiology Spectrum 2023-06-15 [PMID: 37125906]

Bioinformatics

• Gene Symbol: CLEC1B
• Uniprot:
  • AAF36777()