cleavage stimulation factor Antibody

Images

 
Western Blot: cleavage stimulation factor Antibody [NBP2-15913] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: cleavage stimulation factor Antibody [NBP2-15913] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: cleavage stimulation factor Antibody [NBP2-15913] - Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.81 mg/ml

Order Details

cleavage stimulation factor Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human cleavage stimulation factor. The exact sequence is proprietary.
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CSTF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (98%), Xenopus tropicalis (98%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.81 mg/ml
Purity
Immunogen affinity purified

Alternate Names for cleavage stimulation factor Antibody

  • CF-1 50 kDa subunit
  • Cleavage stimulation factor 50 kDa subunit
  • cleavage stimulation factor subunit 1
  • cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kD
  • cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa
  • CSTF 50 kDa subunit
  • CstF-50
  • CstFp50

Background

This gene encodes one of three subunits which combine to form cleavage stimulation factor (CSTF). CSTF is involved in the polyadenylation and 3'end cleavage of pre-mRNAs. Similar to mammalian G protein beta subunits, this protein contains transducin-like repeats. Several transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CSTF1
Uniprot