Claudin-8 Antibody (474210) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with human Claudin-8 was stained with Mouse Anti-Human Claudin-8 Monoclonal Antibody (Catalog # MAB5275, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Caki-2 human clear cell carcinoma epithelial cell line was stained with Mouse Anti-Human Claudin-8 Monoclonal Antibody (Catalog # MAB5275, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready, Flow
• Clone: 474210
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Claudin-8 Antibody (474210) [Unconjugated] Summary

• Immunogen: HEK293 human embryonic kidney cell line transfected with human Claudin-8
  Met1-Val225 (Ser151Pro)
  Accession # P56748
• Specificity: Detects human Claudin‑8. Stains human Claudin-8 transfectants but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLDN8
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Claudin-8 Antibody (474210) [Unconjugated]

• claudin 8
• Claudin8
• Claudin-8
• CLDN8
• human CLDN8 gene for claudin-810claudin-8

Background

Claudin-8 is a 225 amino acid (aa), 25 kDa four-transmembrane protein in the Claudin family of tight junction proteins. It is selectively expressed in tight junctions in the kidney thin descending limb, distal tubule and collecting duct, but is also found in liver and intestinal tight junctions. It is thought to function as an ion barrier protein. The extracellular portions of human Claudin-8 share 86% and 89% aa identity with corresponding portions of mouse and rat Claudin-8, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLDN8
• Uniprot:
  • P56748()