Human Claudin-5 ELISA Kit (Colorimetric)

ELISA: Human Claudin-5 ELISA Kit (Colorimetric) [NBP2-75332] - Samples were spiked with high concentrations of Human Claudin-5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Claudin-5 ELISA Kit (Colorimetric) [NBP2-75332] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL

Human Claudin-5 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human CLDN5 in samples. No significant cross-reactivity or interference between Human CLDN5 and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.35%
• Intra-Assay: CV% < 4.82%
• Spike Recovery: 93-109%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CLDN5

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Claudin-5 ELISA Kit (Colorimetric)

• AWAL
• AWALTransmembrane protein deleted in VCFS
• BEC1
• claudin 5
• Claudin5
• Claudin-5
• CLDN5
• CPETRL1
• TMDVCF
• TMVCF1
• TMVCFTMDVCF
• transmembrane protein deleted in velocardiofacial syndrome

Background

The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the Claudins, Occludin and junction adhesion molecule (JAM). Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is highly restricted to specific regions of different tissues and may have an important role in transcellular transport through tight junctions. Claudin-5 is expressed in the endothelial junctions of the rat liver and in junctions of acinar cells of the pancreas. Human claudin-5 is abundantly expressed in adult lung, heart and skeletal muscle and is deleted in patients with velocardiofacial syndrome, which is characterized by cleft palate, facial dysmorphology and conotruncal heart defects.The human claudin-5 gene maps to chromosome 22q11.21.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Claudin-5 ELISA Kit (NBP2-75332)(3)

Publications using NBP2-75332

• Hochman E, Taler M, Flug R et al. Serum claudin-5 levels among patients with unipolar and bipolar depression in relation to the pro-inflammatory cytokine tumor necrosis factor-alpha levels Brain, behavior, and immunity 2023-01-24 [PMID: 36706845] (ELISA, Human)
  
  Details:
  Serum samples
• Choi J, Kim J, Park M, Lee H Effects of Flavonoid-Rich Orange Juice Intervention on Major Depressive Disorder in Young Adults: A Randomized Controlled Trial Nutrients 2022-12-28 [PMID: 36615801] (ELISA)
• Takanori Iwamoto, Takaya Kitano, Naoki Oyama, Yoshiki Yagita, Quan Jiang Predicting hemorrhagic transformation after large vessel occlusion stroke in the era of mechanical thrombectomy PLoS ONE 2021-08-16 [PMID: 34398910]

FAQs for Claudin-5 ELISA Kit (NBP2-75332). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CLDN5