Claudin-5 Antibody

Product Discontinued

Claudin-5 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 171-220 of Human Claudin-5.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CLDN5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of Claudin 5 (S201) pAb in extracts from A549 cells.
  Immunohistochemistry (IHC) analyzes of Claudin 5 (S201) pAb in paraffin-embedded human brain tissue.
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Claudin-5 Overexpression Lysate

Reactivity Notes

Human, Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Claudin-5 Antibody

• AWAL
• AWALTransmembrane protein deleted in VCFS
• BEC1
• claudin 5
• Claudin5
• Claudin-5
• CLDN5
• CPETRL1
• TMDVCF
• TMVCF1
• TMVCFTMDVCF
• transmembrane protein deleted in velocardiofacial syndrome

Background

The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the Claudins, Occludin and junction adhesion molecule (JAM). Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is highly restricted to specific regions of different tissues and may have an important role in transcellular transport through tight junctions. Claudin-5 is expressed in the endothelial junctions of the rat liver and in junctions of acinar cells of the pancreas. Human claudin-5 is abundantly expressed in adult lung, heart and skeletal muscle and is deleted in patients with velocardiofacial syndrome, which is characterized by cleft palate, facial dysmorphology and conotruncal heart defects.The human claudin-5 gene maps to chromosome 22q11.21.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Claudin-5 Antibody (NB100-91713)(2)

Publications using NB100-91713

• Ding R, Chen Y, Yang S et al. Blood-brain barrier disruption induced by hemoglobin in vivo: Involvement of up-regulation of nitric oxide synthase and peroxynitrite formation. Brain Res. 2014-05-09 [PMID: 24814387] (IHC-P, WB, Rat)
• Madlambayan GJ, Butler JM, Hosaka K et al. Bone marrow stem progenitor cell contribution to neovasculogenesis is dependent on model system with SDF-1 as a permissive trigger. Blood :blood-2009-2003-211342. 2009-01-01 [PMID: 19717647]

FAQs for Claudin-5 Antibody (NB100-91713). (Showing 1 - 1 of 1 FAQs).

1. We are looking for a Claudin 5 antibody to react against the rat antigen. Has this antibody been cited in any paper/manuscripts?
   • We do not have any peer-reviewed references for NB100-91713 that have been discovered by our lab at this moment. If any references are found we will be sure to include them on the datasheet for review.

Bioinformatics

• Gene Symbol: CLDN5
• Entrez:
  • 7122(Human)
  • 12741(Mouse)
  • 65131(Rat)
• Uniprot:
  • O00501(Human)