Claudin-3 Overexpression Lysate (Native)


Western Blot: Claudin 3 Overexpression Lysate (Adult Normal) [NBL1-09245] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Claudin 3.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

Claudin-3 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC204882 and based on accession number NM_001306. The protein contains a C-terminal DDK tag.
Homo sapiens claudin 3 (CLDN3), mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
23.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for Claudin-3

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Claudin-3 Overexpression Lysate (Native)

  • C7orf1
  • claudin 3
  • Claudin3
  • claudin-3
  • CLDN3
  • Clostridium perfringens enterotoxin receptor 2
  • CPE-R 2
  • CPE-R2
  • CPETR2
  • CPETR2CPE-receptor 2
  • HRVP1
  • Rat ventral prostate.1 protein homolog
  • RVP1
  • ventral prostate.1 protein homolog
  • ventral prostate.1-like protein


Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Claudin-3 Lysate (NBL1-09245) (0)

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Contact Information

Product PDFs


Gene Symbol CLDN3

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