Human Claudin-3 ELISA Kit (Colorimetric)

ELISA: Human Claudin-3 ELISA Kit (Colorimetric) [NBP2-75328] - Samples were spiked with high concentrations of Human Claudin-3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Claudin-3 ELISA Kit (Colorimetric) [NBP2-75328] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL

Human Claudin-3 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human CLDN3 in samples. No significant cross-reactivity or interference between Human CLDN3 and analogues was observed.
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.41%
• Intra-Assay: CV% < 5.09%
• Spike Recovery: 85-101%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CLDN3

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Claudin-3 ELISA Kit (Colorimetric)

• C7orf1
• claudin 3
• Claudin3
• Claudin-3
• CLDN3
• Clostridium perfringens enterotoxin receptor 2
• CPE-R 2
• CPE-R2
• CPETR2
• CPETR2CPE-receptor 2
• HRVP1
• Rat ventral prostate.1 protein homolog
• RVP1
• ventral prostate.1 protein homolog
• ventral prostate.1-like protein

Background

The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the Claudins, Occludin and junction adhesion molecule (JAM). Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is highly restricted to specfic regions of different tissues and variations of Claudin expression may have an important role in transcellular transport through tight junctions. In rat liver, claudin-3 is uniformly expressed, whereas in the pancreas, claudin-3 is expressed in junctions of the duct epithelia and junctions of acinar cells. Claudin-3 binds the peptide toxin Clostridium perfringens enterotoxin (CPE) at the cell surface via the second extracellular loop of claudin-3. The gene encoding human claudin-3 maps to chromosome 7q11.23.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Claudin-3 ELISA Kit (NBP2-75328)(2)

Publications using NBP2-75328

• Arango-GonzAlez A, Lara-Guzman O, Escobar J et al. Putative intestinal permeability markers do not correlate with cardiometabolic health and gut microbiota in humans, except for peptides recognized by a widely used zonulin ELISA kit Nutrition, Metabolism and Cardiovascular Diseases 2022-10-01 [PMID: 36462977] (ELISA)
• Sahinovic A, Irwin C, Doohan PT et al. Effects of Cannabidiol on Exercise Physiology and Bioenergetics: A Randomised Controlled Pilot Trial Sports medicine - open 2022-03-02 [PMID: 35235092] (ELISA)

FAQs for Claudin-3 ELISA Kit (NBP2-75328). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CLDN3