Recombinant Human Claudin-1 GST (N-Term) Protein

SDS-Page: Recombinant Human Claudin-1 Protein [H00009076-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

Product Discontinued

Recombinant Human Claudin-1 GST (N-Term) Protein Summary

• Description: A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-211 of Human CLDN1
  
  

  Source: Wheat Germ (in vitro)

  Amino Acid Sequence: MANAGLQLLGFILAFLGWIGAIVSTALPQWRIYSYAGDNIVTAQAMYEGLWMSCVSQSTGQIQCKVFDSLLNLSSTLQATRALMVVGILLGVIAIFVATVGMKCMKCLEDDEVQKMRMAVIGGAIFLLAGLAILVATAWYGNRIVQEFYDPMTPVNARYEFGQALFTGWAAASLCLLGGALLCCSCPRKTTSYPTPRPYPKPAPSSGKDYV

• Preparation Method: in vitro wheat germ expression system
• Details of Functionality: This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
• Source: Wheat germ
• Protein/Peptide Type: Recombinant Protein
• Gene: CLDN1
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • Western Blot
• Theoretical MW: 48.95 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human Claudin-1 GST (N-Term) Protein

• claudin 1
• Claudin1
• Claudin-1
• CLD1
• CLDN1
• ILVASCSenescence-associated epithelial membrane protein
• SEMP1
• SEMP1senescence-associated epithelial membrane protein 1

Background

CLDN1( AAH12471, 1 a.a. - 212 a.a.) recombinant protein with GST.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Claudin-1 Full Length Recombinant Protein (H00009076-P01)(5)

Publications using H00009076-P01

• Morishima A, Hirano T, Nishikawa H et al. Comprehensive exploration of autoantibody in Behcets disease: A novel autoantibody to claudin-1, an essential protein for tight junctions, is identified. Joint Bone Spine. 2014-04-29 [PMID: 24792642]
• Broussard EK, Kim R, Wiley JC et al. Identification of putative immunologic targets for colon cancer prevention based on conserved gene expression from pre-invasive to malignant lesions. Cancer Prev Res (Phila Pa) 2013-06-10 [PMID: 23682078]
• Lim TS, Vedula SR, Hunziker W et al. Kinetics of Adhesion Mediated by Extracellular Loops of Claudin-2 as Revealed by Single Molecule Force Spectroscopy. J Mol Biol;381(3):681-91. 2008-09-05 [PMID: 18635194]
• Lim TS, Vedula SR, Huic S et al. Probing Effects of pH change on Dynamic Response of Claudin-2 Mediated Adhesion Using Single Molecule Force Spectroscopy. Exp Cell Res 2008-06-01 [PMID: 18602630]
• Lim TS, Vedula SR, Kausalya PJ et al. Single-molecular-level study of claudin-1-mediated adhesion. Langmuir;24(2):490-5. 2008-01-15 [PMID: 18095722]

Bioinformatics

• Gene Symbol: CLDN1