Clathrin Heavy Chain 1/CHC17 Antibody [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 594

Clathrin Heavy Chain 1/CHC17 Antibody [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human Clathrin Heavy Chain 1/CHC17
  Trp1521-Met1675
  Accession # Q00610
• Specificity: Detects human Clathrin Heavy Chain 1/CHC17 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CHC-22 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Clathrin Heavy Chain 1/CHC17 Antibody [Alexa Fluor® 594]

• CHC
• CHC17
• Clathrin Heavy Chain 1
• Clathrin heavy chain on chromosome 17
• clathrin, heavy chain (Hc)
• clathrin, heavy chain
• clathrin, heavy polypeptide (Hc)
• clathrin, heavy polypeptide-like 2
• CLH17
• CLH-17
• CLTC
• CLTCL2
• Hc
• KIAA0034

Background

Clathrin Heavy Chain 1/CHC17 (clathrin heavy chain 17; also CHC, CLTCL2 and CLTC) is a 170‑180 kDa member of the clathrin heavy chain family of molecules. It is widely expressed, and serves at least two functions. First, it interacts with TACC3 and chTOG to form a complex that crosslinks microtubules making up K‑fibers, and second, three Clathrin Heavy Chain 1/CHC17 molecules form a triskelion (Greek for "3 legged") structure with three clathrin light chains to create the polyhedral coat of coated pits. Human Clathrin Heavy Chain 1/CHC17 is 1675 amino acids (aa) in length. It contains an N‑terminal domain that mediates protein‑protein interactions (aa 2‑479), a flexible linker region (aa 480‑523), a distal segment (aa 524‑638), a proximal region that interacts noncovalently with one CHC light chain (aa 639‑1549), and a trimerization domain that mediates the formation of a noncovalent triskelion structure (aa 1550‑1675). Although the predicted MW of Clathrin Heavy Chain 1/CHC17 is 191 kDa, it runs anomalously at 180 kDa in SDS‑PAGE. Multiple utilized phosphorylation and acetylation sites exist. There are multiple potential splice forms. One shows a four aa insertion after Lys83, a second shows a seven aa insertion after Asp1611, and a third contains a four aa substitution for aa 1636‑1675. Over aa 1521‑1675, human and mouse Clathrin Heavy Chain 1/CHC17 are identical in aa sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.