CIZ1 Antibody

Western Blot: CIZ1 Antibody [NB100-74623] - Detection of Human CIZ1 on HeLa whole cell lysate. CIZ1 was also immunoprecipitated by rabbit anti-CIZ1 antibody NB100-74624.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CIZ1 Antibody Summary

• Description: Novus Biologicals Rabbit CIZ1 Antibody (NB100-74623) is a polyclonal antibody validated for use in WB and IP. Anti-CIZ1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 325 and 375 of human CDKN1A interacting zinc finger protein 1 using the numbering given in entry NP_036259.2 (GeneID 25792).
• Localization: Cytoplasmic and Nuclear
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CIZ1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CIZ1 Antibody

• CDKN1A interacting zinc finger protein 1
• CDKN1A-interacting zinc finger protein 1
• cip1-interacting zinc finger protein
• LSFR1ZNF356Nuclear protein NP94
• NP94
• Zinc finger protein 356

Background

CIZ1 (Cip1 interacting zinc-finger protein) was identified as a protein that associates with the cell-cycle inhibiting protein, p21 (Cip1/Waf1). CIZ1 is a member of the matrin 3 protein family and has been shown to bind DNA consensus sequences and co-localize with PCNA during replication. In breast cancer cells, CIZ1 was shown to associate with Cdk2 and dynein light chain 1 (DLC1), a cytoskeletal signaling protein involved in cell cycle regulation and tumorigenesis. Studies of the role of CIZ1 in breast tumorigenesis revealed that CIZ1 is an estrogen-responsive gene that functions as a coactivator of the estrogen receptor. Targeted depletion of CIZ1 inhibits entry into S-phase. This finding along with evidence that CIZ1 interacts with multiple cell cycle regulatory proteins and localizes to DNA replication foci, indicates that CIZ1 plays a critical role in the regulation of DNA replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CIZ1 Antibody (NB100-74623)(1)

Publication using NB100-74623

• Coverley D, Higgins G, West D et al. A quantitative immunoassay for lung cancer biomarker CIZ1b in patient plasma. Clin. Biochem. 2016-11-17 [PMID: 27867087] (WB)

Bioinformatics

• Gene Symbol: CIZ1
• Entrez:
  • 25792(Human)
• Uniprot:
  • NP_036259.2(Human)