Recombinant Human Citidine Deaminase Protein

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SDS-Page: Recombinant Human Citidine Deaminase Protein [NBP1-49306] - 15 % SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human Citidine Deaminase Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-146 of Human Citidine Deaminase

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAQKRPACTL KPECVQQLLV CSQEAKQSAY CPYSHFPVGA ALLTQEGRIF KGCNIENACY PLGICAERTA IQKAVSEGYK DFRAIAIASD MQDDFISPCG ACRQVMREFG TNWPVYMTKP DGTYIVMTVQ ELLPSSFGPE DLQKTQ

Details of Functionality
Specific activity:> 3.5 unit/mg. One unit of activity was defined as the amount required to deaminate 1.0 umole of cytidine per min at pH 7.5 at 25C.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
CDA
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
18.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl Buffer (pH 8.0) containing 1 mM DTT, 2 mM EDTA, 100 mM NaCl, 40% Glycerol
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human Citidine Deaminase Protein

  • CDDCytidine aminohydrolase
  • cytidine deaminase
  • cytosine nucleoside deaminase
  • EC 3.5.4.5
  • small cytidine deaminase

Background

CDA (Cytidine deaminase) is an enzyme that scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. This protein is one of several deaminases responsible for maintaining the cellular pyrimidine pool. The protein also catalyzes the deamination of chemotherapeutic cytosine nucleoside analogs such as Ara-C and 5-azacytidine, which results in the loss of their cytotoxic and antitumor function. It can form homotetramers and is mainly expressed in granulocytes. Recombinant human CDA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol CDA