CISH/CIS-1 Antibody

Western Blot: CISH/CIS-1 Antibody [H00001154-D01P] - Analysis of CISH expression in transfected 293T cell line by CISH polyclonal antibody.Lane 1: CISH transfected lysate(28.70 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: CISH/CIS-1 Antibody [H00001154-D01P] - Analysis of antibody to CISH on HeLa cell. Antibody concentration 10 ug/ml.

Product Discontinued

CISH/CIS-1 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: CISH (NP_659508.1, 1 a.a. - 258 a.a.) full-length human protein. MVLCVQGPRPLLAVERTGQRPLWAPSLELPKPVMQPLPAGAFLEEVAEGTPAQTESEPKVLDPEEDLLCIAKTFSYLRESGWYWGSITASEARQHLQKMPEGTFLVRDSTHPSYLFTLSVKTTRGPTNVRIEYADSSFRLDSNCLSRPRILAFPDVVSLVQHYVASCTADTRSDSPDPAPTPALPMPKEDAPSDPALPAPPPATAVHLKLVQPFVRRSSARSLQHLCRLVINRLVADVDCLPLPRRMADYLRQYPFQL
• Specificity: CISH - cytokine inducible SH2-containing protein,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CISH
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CISH/CIS-1 Antibody

• CIS-1
• CIS-1cytokine-inducible SH2-containing protein
• CIScytokine-inducible inhibitor of signaling type 1B
• CISH
• cytokine inducible SH2-containing protein
• G18Protein G18
• SOCS
• Suppressor of cytokine signaling

Background

The protein encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CISH
• Entrez:
  • 1154(Human)
• Uniprot:
  • NP_659508.1(Human)