CISD1 Overexpression Lysate (Native)

Images

 
Western Blot: CISD1 Overexpression Lysate (Adult Normal) [NBL1-09214] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CISD1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CISD1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203308 and based on accession number NM_018464. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens CDGSH iron sulfur domain 1 (CISD1), mRNA.
Gene
CISD1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for CISD1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CISD1 Overexpression Lysate (Native)

  • C10orf70
  • CDGSH iron sulfur domain 1
  • CDGSH iron sulfur domain-containing protein 1
  • CDGSH iron-sulfur domain-containing protein 1
  • chromosome 10 open reading frame 70
  • MDS029
  • MGC14684
  • mitoNEET
  • ZCD1
  • zinc finger CDGSH-type domain 1
  • zinc finger, CDGSH-type domain 1

Background

CISD1 is a member of the CDGSH domain-containing family and may play a role in the regulation of mitochondrial oxidative capacity (Wiley et al., 2007, 2007 [PubMed 17376863] [PubMed 17584744]).[supplied by OMIM]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CISD1