CIRBP Antibody - BSA Free

Western Blot: CIRBP Antibody [NBP2-15905] - Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: CIRBP Antibody [NBP2-15905] - A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CIRBP protein stained by CIRBP antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: CIRBP Antibody [NBP2-15905] - Immunohistochemical analysis of paraffin-embedded U373 xenograft, using antibody at 1:500 dilution.

• Reactivity: Hu, Mu, Rt, Bv, Ch, Pm, RM
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CIRBP Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human CIRBP. The exact sequence is proprietary.
• Localization: Nucleus; nucleoplasm
• Predicted Species: Mouse (94%), Rat (94%), Chicken (90%), Primate (97%), Rhesus Macaque (100%), Bovine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CIRBP
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:500-1:3000
• Application Notes: WB: Initial test suggest that Mouse protein may be difficult to detect. Immunoprecipitation Assay-dependent dilution
• Theoretical MW: 19 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (97%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for CIRBP Antibody - BSA Free

• A18 hnRNP
• A18HNRNP
• CIRPcold-inducible RNA-binding protein
• cold inducible RNA binding protein
• cold inducible RNA-binding protein
• glycine-rich RNA binding protein
• Glycine-rich RNA-binding protein CIRP

Background

Seems to play an essential role in cold-induced suppression of cell proliferation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for CIRBP Antibody (NBP2-15905)(1)

Publication using NBP2-15905

• Kim SY, Ban HJ, Lee S, Jin HJ. Regulation of CIRP by genetic factors of SP1 related to cold sensitivity Frontiers in Immunology 2022-09-16 [PMID: 36189232]

Bioinformatics

• Gene Symbol: CIRBP
• Uniprot:
  • Q14011(Human)