CIRBP Antibody (3V9S7)

Western Blot: CIRBP Antibody (3V9S7) [NBP3-15745] - Analysis of extracts of various cell lines, using CIRBP antibody (NBP3-15745) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Immunohistochemistry: CIRBP Antibody (3V9S7) [NBP3-15745] - Immunohistochemistry analysis of CIRBP in paraffin-embedded human lung tissue using CIRBP Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunocytochemistry/ Immunofluorescence: CIRBP Antibody (3V9S7) [NBP3-15745] - Confocal imaging of paraffin-embedded Human breast cancer tissue using CIRBP Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Immunohistochemistry: CIRBP Antibody (3V9S7) [NBP3-15745] - Immunohistochemistry analysis of CIRBP in paraffin-embedded human thyroid tissue using CIRBP Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: CIRBP Antibody (3V9S7) [NBP3-15745] - Immunohistochemistry analysis of CIRBP in paraffin-embedded human pancreas tissue using CIRBP Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: 3V9S7
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

CIRBP Antibody (3V9S7) Summary

• Description: Novus Biologicals Rabbit CIRBP Antibody (3V9S7) (NBP3-15745) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human CIRBP (Q14011). MASDEGKLFVGGLSFDTNEQSLEQVFSKYGQISEVVVVKDRETQRSRGFGFVTFENIDDAKDAMMAMNGKSVDGRQIRVDQAGKSSDNRSRGYRGGSAGGRGFFRGGRGRGRGFSRGGGDRGYGGNRFESRSGGYGGSRDYYSSRSQSGGYSDRSSGGSYRDSYDSYATHNE
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CIRBP
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for CIRBP Antibody (3V9S7)

• A18 hnRNP
• A18HNRNP
• CIRPcold-inducible RNA-binding protein
• cold inducible RNA binding protein
• cold inducible RNA-binding protein
• glycine-rich RNA binding protein
• Glycine-rich RNA-binding protein CIRP

Background

Seems to play an essential role in cold-induced suppression of cell proliferation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CIRBP