CIB1 Antibody

Western blot shows lysates of human spleen tissue, mouse kidney tissue, and rat kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat CIB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7557) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CIB1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

CIB1 Antibody Summary

• Immunogen: E. coli-derived recombinant human CIB1
  Gly2-Leu191
  Accession # Q99828
• Specificity: Detects human, mouse, and rat CIB1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CIB1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CIB1 Antibody

• calcium and integrin binding 1 (calmyrin)
• Calcium- and integrin-binding protein
• Calmyrin
• CIB1
• CIBcalcium and integrin binding protein
• CIBP
• DNA-dependent protein kinase interacting protein
• DNA-PKcs-interacting protein
• Kinase-interacting protein
• KIP
• KIP1
• KIPcalcium and integrin binding, protein kinase interacting protein
• PRKDCIP
• SIP2-28
• SIP2-28calcium and integrin-binding protein 1
• Snk interacting protein 2-28
• SNK-interacting protein 2-28

Background

CIB1 (Calcium and Integrin-Binding protein 1; also calmyrin, KIP and CIBP) is a 22-23 kDa, Ca⁺⁺-binding member of the CIB family of proteins. It is widely expressed, being found in neurons, platelets/megakaryocytes, skeletal muscle myocytes and lymphocytes. CIB1 is associated with the cytosolic side of the plasma membrane, and has multiple binding partners, including InsP₃R, GPIIb/ alpha IIb, presenilin 2, and NBR1 plus FEZ. When CIB1 binds InsP₃R, this ER-embedded receptor is both activated, and later desensitized to subsequent ligand binding. Relative to GPIIb, CIB1 interaction with the alpha IIb beta ₃ integrin on platelets following thrombin exposure appears to inhibit integrin activation, thus providing a tight control on subsequent platelet binding to fibrinogen. Human CIB1 is 191 amino acids (aa) in length. It contains a utilized myristoylation site at Gly2, followed by two EF-hand domains (aa 103-183). CIB1 is suggested to act as a monomer. There is at least one potential isoform variant that shows a 40 aa insertion after Lys29. Full-length human CIB1 (aa 1-191) shares 94% aa sequence identity with mouse CIB1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CIB1 Antibody (AF7557)(1)

Publication using AF7557

• CJ Wu, X Li, CL Sommers, K Kurima, S Huh, G Bugos, L Dong, W Li, AJ Griffith, LE Samelson Expression of a TMC6-TMC8-CIB1 heterotrimeric complex in lymphocytes is regulated by each of the components J. Biol. Chem., 2020-09-11;0(0):. 2020-09-11 [PMID: 32917726] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: CIB1
• Uniprot:
  • Q99828()