CIB1 Antibody [Alexa Fluor® 532]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 532

CIB1 Antibody [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant human CIB1
  Gly2-Leu191
  Accession # Q99828
• Specificity: Detects human, mouse, and rat CIB1 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CIB1 Antibody [Alexa Fluor® 532]

• calcium and integrin binding 1 (calmyrin)
• Calcium- and integrin-binding protein
• Calmyrin
• CIB1
• CIBcalcium and integrin binding protein
• CIBP
• DNA-dependent protein kinase interacting protein
• DNA-PKcs-interacting protein
• Kinase-interacting protein
• KIP
• KIP1
• KIPcalcium and integrin binding, protein kinase interacting protein
• PRKDCIP
• SIP2-28
• SIP2-28calcium and integrin-binding protein 1
• Snk interacting protein 2-28
• SNK-interacting protein 2-28

Background

CIB1 (Calcium and Integrin-Binding protein 1; also calmyrin, KIP and CIBP) is a 22-23 kDa, Ca⁺⁺-binding member of the CIB family of proteins. It is widely expressed, being found in neurons, platelets/megakaryocytes, skeletal muscle myocytes and lymphocytes. CIB1 is associated with the cytosolic side of the plasma membrane, and has multiple binding partners, including InsP₃R, GPIIb/ alpha IIb, presenilin 2, and NBR1 plus FEZ. When CIB1 binds InsP₃R, this ER-embedded receptor is both activated, and later desensitized to subsequent ligand binding. Relative to GPIIb, CIB1 interaction with the alpha IIb beta ₃ integrin on platelets following thrombin exposure appears to inhibit integrin activation, thus providing a tight control on subsequent platelet binding to fibrinogen. Human CIB1 is 191 amino acids (aa) in length. It contains a utilized myristoylation site at Gly2, followed by two EF-hand domains (aa 103-183). CIB1 is suggested to act as a monomer. There is at least one potential isoform variant that shows a 40 aa insertion after Lys29. Full-length human CIB1 (aa 1-191) shares 94% aa sequence identity with mouse CIB1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.