cIAP-1/HIAP-2 Antibody

Product Discontinued

cIAP-1/HIAP-2 Antibody Summary

• Immunogen: Full-length recombinant human cAIP1 was used as immunogen, GenBank no. 2497238 RP/human; gel purified; human
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BIRC2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:1000-1:5000~
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:1000-1:2000~
• Application Notes: 1. cIAP1 has 618 amino acids according to GenBank no. and migrates at ~69kDaonSDS-PAGE.2. Observed molecular weights may vary as genes generally produce, by alternative splicing, multiple transcripts all with introns, putatively encoding multipledifferent protein isoforms. Additionally, proteins may undergo post translational modifications including phosphorylation, glycosylation and cleavage which can result in differential molecular weights. Protein modifications can vary between cell and tissue type, and proteins can migrate as one or multiple bands depending on the isoform expressed as well as post-translational modifications. Users are encourage to refer to the NCBI AceView. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30054903).

Reactivity Notes

Dog,Gerbil,Human,Mouse,Rat

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50 ul neat whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for cIAP-1/HIAP-2 Antibody

• API1Hiap-2
• apoptosis inhibitor 1
• baculoviral IAP repeat containing 2
• baculoviral IAP repeat-containing 2
• baculoviral IAP repeat-containing protein 2
• BIRC2
• cIAP1
• c-IAP1
• cIAP-1
• hIAP2
• HIAP-2
• IAP homolog B
• IAP2
• IAP-2
• Inhibitor of apoptosis protein 2
• MIHB
• MIHBHIAP2
• NFR2-TRAF signalling complex protein
• RING finger protein 48
• RNF48hiap-2
• TNFR2-TRAF-signaling complex protein 2

Background

cIAP1 (HIAP2, MIHB) is a member of the family of inhibitor of apoptosis proteins (IAP). IAPs suppress mitochondria-dependent and -independent apoptosis by binding to and inhibiting caspases through their BIR domains (reviewed in Liston et al, 2003; Wright and Duckett, 2005). Resistance towards apoptosis is a hallmark of cancer cells, and overexpression of IAPs can contribute to the development of cancer though inhibiting apoptosis. In addition to at least one BIR domain, some IAP members also have a RING-type finger motif at their carboxyl-terminal. The RING finger domain of several IAPs, including cIAP2, have E3 ubiquitin ligase activity and target the degradation of Smac/DIABLO through ubqiuitination. Smac/DIABLO is a death inducer and functions by inhibiting IAP-caspase interactions, thereby promoting apoptosis. Degradation of cell death inducers like Smac/DIABLO is thought to be a conserved mechanism by which IAPs enhance their anti-apoptotic activity, thereby promoting cell survival. The IAPs, including cIAP1, have widespread tissue protein expression, with expression levels and subcellular localization patterns differing depending on the cell lineage (see Vischioni et al. 2005 for a comprehensive study). IMG-5716 recognizes cIAP1, human cIAP1 is a 618 amino acid protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for cIAP-1/HIAP-2 Antibody (NBP2-27190)(2)

Publications using NBP2-27190

• Twayana KS, Chaudhari N, Ravanan P. Prolonged lipopolysaccharide exposure induces transient immunosuppression in BV2 microglia. J Cell Physiol 2019-02-01 [PMID: 30054903] (ICC/IF, Mouse)
• Chui YL, Ma CH, Li W et al. Anti-apoptotic protein BRE/BRCC45 attenuates apoptosis through maintaining the expression of caspase inhibitor XIAP in mouse Lewis lung carcinoma D122 cells. Apoptosis. 2014-01-07 [PMID: 24395041] (WB, Mouse)
  
  Details:
  Retrovirus and plasmid transfection: pBABEneo-BREV5 retroviruses were generated by the co-transfection of retroviral expression vector pBABEneo-BREV5 and the packaging vector pCL-Ampho to the packaging cell line HEk293T.

Bioinformatics

• Gene Symbol: BIRC2
• Uniprot:
  • Q13490()