Home » Chorionic Gonadotropin beta Chain (hCG beta) » Chorionic Gonadotropin beta Chain (hCG beta) Antibodies » Chorionic Gonadotropin beta Chain (hCG beta) Antibody (INN-hCG-53) [Biotin]

Chorionic Gonadotropin beta Chain (hCG beta) Antibody (INN-hCG-53) [Biotin]

 Submit a Review

Images

 
There are currently no images for hCG holo C2 Antibody (NB100-63167B).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
INN-hCG-53
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NB100-63167AF405
Alexa Fluor 488
NB100-63167AF488
Alexa Fluor 647
NB100-63167AF647
Alexa Fluor 700
NB100-63167AF700
DyLight 350
NB100-63167UV
DyLight 405
NB100-63167V
DyLight 488
NB100-63167G
DyLight 550
NB100-63167R
DyLight 650
NB100-63167C
DyLight 680
NB100-63167FR
DyLight 755
NB100-63167IR
FITC
NB100-63167F
HRP
NB100-63167H
Unconjugated
NB100-63167
100% Guarantee on every product

Chorionic Gonadotropin beta Chain (hCG beta) Antibody (INN-hCG-53) [Biotin] Summary

Immunogen
Made to Human hCG holo C2
Localization
Secreted
Specificity
NB100-63167 recognises epitope c2 on hCG holo C2 . It does not recognise free hCG subunits.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CGB3
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Readout System

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Chorionic Gonadotropin beta Chain (hCG beta) Antibody (INN-hCG-53) [Biotin]

  • CGB
  • CGB3
  • CGB3choriogonadotropin subunit beta
  • CGB5
  • CGB7
  • CGB8
  • CG-Beta
  • Choriogonadotropin Subunit Beta
  • Chorionic gonadotrophin chain beta
  • chorionic gonadotropin beta 3 subunit
  • Chorionic Gonadotropin beta Chain
  • chorionic gonadotropin beta subunit
  • chorionic gonadotropin, beta polypeptide
  • hCGB

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Chromogranin B
Antibody
NBP1-80782
Western Blot: Chromogranin B Antibody [NBP1-80782] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Adrenal Gland tissue
Immunocytochemistry/Immunofluorescence: Chromogranin B Antibody [NBP1-80782] - Staining of human cell line SH-SY5Y shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Chromogranin B Antibody [NBP1-80782] - Staining of human colon shows strong cytoplasmic positivity in enteroendocrine cells.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Serpin A6/Cortisol
Binding Globulin Anti ...
MAB3838
Western blot shows lysates of rat olfactory bulb tissue, rat liver tissue, rat brain (cerebellum) tissue, and rat brain (hippocampus) tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Rat Serpin A6 Monoclonal Antibody (Catalog # MAB3838) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin A6 at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Serpin A6 was detected in perfusion fixed frozen sections of rat brain (preoptic nucleus) using Mouse Anti-Rat Serpin A6 Monoclonal Antibody (Catalog # MAB3838) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Species: Rt
Applications: WB, IHC
LH beta Antibody
AF8016
Western blot shows lysates of human pituitary gland tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human LH beta  Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8016) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LH beta at approximately 17-19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
LH beta  was detected in immersion fixed paraffin-embedded sections of human pituitary adenoma using Sheep Anti-Human LH beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8016) at 1 µg/mL overnight at4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter­stained with hematoxylin (blue). Specific staining was localized to cytoplasm of pituitary cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Species: Hu
Applications: WB, IHC
Chorionic
Gonadotropin alpha Ch ...
MAB4169
Chorionic Gonadotropin, alpha  Chain ( alpha  HCG) was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human Chorionic Gonadotropin, alpha  Chain ( alpha  HCG) Monoclonal Antibody (Catalog # MAB4169) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(1 Review)
Species: Hu
Applications: WB, IHC
PLOD1 Antibody
NBP2-38770
Western Blot: PLOD1 Antibody [NBP2-38770] - Analysis in human cell line U-87 MG.
Western Blot: PLOD1 Antibody [NBP2-38770] - SC65 directly interacts with lysyl-hydroxylase 1 (LH1). Western blot of primary calvarial osteoblast and skin fibroblast lysates from WT and Sc65KO 3 day-old mice (N = 2) showing significantly decreased levels of LH1 protein in Sc65KO samples. Densitometric quantification of LH1 protein normalized to beta-actin from the western blot shown above (*p<0.05; error bars represent SD). All experiments were performed at least 3 times. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pgen.1006002), licensed under a CC-BY licence.
Western Blot: PLOD1 Antibody [NBP2-38770] - Analysis in human cell line U-251 MG and human cell line RT-4.
(2 Publications)
Species: Hu, Mu
Applications: WB
Chromogranin A
Antibody
NB120-15160
Immunohistochemistry-Paraffin: Chromogranin A Antibody [NB120-15160] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse pancreas using Chromogranin A antibody at a dilution of 1:500. The signal was developed using HRP-conjugated anti-rabbit secondary and DAB reagent which followed counterstaining of the section with hematoxylin. The antibody generated a strong staining in Islets of Langerhans. Also, in the periphery of the cells, the pattern of staining was punctate representing the vesicles with Chromogranin A. Cells of lobules and acini showed a weak cytoplasmic and nuclear staining which is potentially the secreted pool of Chromogranin A.
Immunohistochemistry: Chromogranin A Antibody [NB120-15160] - IHC-P analysis fo human pancreas tissue showing specific staining of Chromogranin A in the Islets of Langerhans (dark brown staining).
Immunocytochemistry/Immunofluorescence: Chromogranin A Antibody [NB120-15160] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Chromogranin A Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
(2 Reviews)
(18 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Cathepsin G
Antibody
NBP2-33498
Immunohistochemistry-Paraffin: Cathepsin G Antibody [NBP2-33498] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.
Immunohistochemistry-Paraffin: Cathepsin G Antibody [NBP2-33498] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.
Immunohistochemistry-Paraffin: Cathepsin G Antibody [NBP2-33498] - Staining of human cerebral cortex shows low expression as expected.
(2 Publications)
Species: Hu
Applications: IHC, IHC-P
CGB2 Antibody (4F8)
H00114336-M02
Western Blot: CGB2 Antibody (4F8) [H00114336-M02] - detection against Immunogen (31.9 KDa).
Species: Hu
Applications: WB, ELISA
Cytosolic
beta-Glucosidase/GBA3 ...
NBP2-00521
Western Blot: GBA3 Antibody (1F1) [NBP2-00521] Analysis of extracts (35ug) from 9 different cell lines by using anti-GBA3 monoclonal antibody.
Immunohistochemistry: Cytosolic beta-Glucosidase/GBA3 Antibody (1F1) [NBP2-00521] - GBA3 Antibody (1F1) [NBP2-00521] - Staining of paraffin-embedded Human prostate tissue using anti-GBA3 mouse monoclonal antibody.
Flow Cytometry: GBA3 Antibody (1F1) [NBP2-00521] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GBA3 antibody, and then analyzed by flow cytometry.
Species: Hu
Applications: WB, Flow, IHC, IHC-P
TRAPPC9 Antibody
NB100-55735
TRAPPC9 Antibody
(1 Publication)
Species: Hu, Mu, Bv, Ca, Ch, Pm
Applications: WB, ICC/IF, IP
Adenine Nucleotide
Translocase 1 Antibod ...
NBP1-59594
Western Blot: Adenine Nucleotide Translocase 1 Antibody [NBP1-59594] - Sample Tissue: Human RPMI-8226 Antibody Dilution: 1.0 ug/ml
Immunohistochemistry: Adenine Nucleotide Translocase 1 Antibody [NBP1-59594] - Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue Observed Staining: Cytoplasmic in cell bodies of pinealocytes and their processes Primary Antibody Concentration: 1:100 Other Working Concentrations: 1/600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec
Western Blot: Adenine Nucleotide Translocase 1 Antibody [NBP1-59594] - RPMI 8226 cell lysate, concentration 0.2-1 ug/ml.
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
ST2/IL-33R
Antibody [Unconjugate
AF523
Recombinant Human IL‑33 induces IFN-gamma secretion in Human Peripheral Blood Mononuclear cells (PBMC) in the presence of 0.25 ng/mL Recombinant Human IL-12 (Catalog # 219-IL)  in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Q-kit (Catalog # DIF50). Under these conditions, IFN-gamma secretion elicited by IL‑33 is neutralized (green line) by increasing concentrations of Goat Anti-Human ST2/IL-33 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF523). The ND50 is typically 0.1-0.6 μg/mL.
Simple Western lane view shows lysates of human kidney tissue and human lung tissue, loaded at 0.2 mg/mL. A specific band was detected for ST2/IL-33 R at approximately 60 kDa (as indicated) using 2 µg/mL of Goat Anti-Human ST2/IL-33 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF523) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
KG‑1a human acute myelogenous leukemia cell line was stained with Goat Anti-Human ST2/IL-33 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF523, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
(1 Review)
(11 Publications)
Species: Hu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, Neut
ACAA2 Antibody
(OTI1C10) - Azide and ...
NBP2-71602
Western Blot: ACAA2 Antibody (OTI1C10) - Azide and BSA Free [NBP2-71602] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAA2.
Immunocytochemistry/Immunofluorescence: ACAA2 Antibody (OTI1C10) - Azide and BSA Free [NBP2-71602] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY ACAA2.
Immunohistochemistry: ACAA2 Antibody (OTI1C10) - Azide and BSA Free [NBP2-71602] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, ICC/IF, IHC
Chromogranin C
Antibody
NBP2-15895
Western Blot: Chromogranin C Antibody [NBP2-15895] - A. 50 ug mouse brain lysate/extract 7.5% SDS-PAGE Chromogranin C antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Chromogranin C Antibody [NBP2-15895] - Paraffin-embedded mouse fore brain. Chromogranin C antibody dilution: 1:500.
Western Blot: Chromogranin C Antibody [NBP2-15895] - Sample (30 ug of whole cell lysate) A: IMR32 B: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
CD5 Antibody
[Unconjugated]
AF1636
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human CD5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1636) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD5 at approximately 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of CEM human T-lymphoblastoid cell line, loaded at 0.2 mg/mL. A specific band was detected for CD5 at approximately 93 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CD5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1636). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Human CD5 Antigen Affinity-purified Polyclonal Antibody enhances IL-2 secretion in human T cells in the presence of sub-optimal amounts of Human CD3 epsilon  Monoclonal Antibody (Catalog # MAB100) and Human CD28 Monoclonal Antibody (Catalog # MAB342), in a dose-dependent manner, as measured using the Quantikine Human IL-2 ELISA Kit (Catalog # D2050). The ED50 for this effect is typically 2‑8 μg/mL.
(1 Publication)
Species: Hu
Applications: WB, Simple Western, Flow, AgAct, CyTOF-ready, ICC
Pea3 Antibody
NBP1-87960
Immunocytochemistry/Immunofluorescence: Pea3 Antibody [NBP1-87960] - Staining of human cell line U-251 MG shows localization to nucleoli. Antibody staining is shown in green. Antibody staining is shown in green.
Immunohistochemistry-Frozen: Pea3 Antibody [NBP1-87960] - Pea3 staining on E11.5 mouse Right Ventricle. Fixed 4% PFA overnight. Blocked with 1% BSA. Primary antibody at 1:40. Secondary antibody at 1:1000, conjugated to Alexa Fluor 488. IHC-Fr image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Pea3 Antibody [NBP1-87960] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.
(1 Review)
(2 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-Fr, IHC-P
Galectin 13
Antibody (PP13/1161)
NBP2-53210
Western Blot: Galectin 13 Antibody (PP13/1161) [NBP2-53210] - Western Blot of (A) Recombinant human Galectin-13 fragment And (B) K562 Cell Lysate using Galeactin-13 MAb (PP13/1161).
Immunohistochemistry-Paraffin: Galectin 13 Antibody (PP13/1161) [NBP2-53210] - Formalin-paraffin human Spleen stained with Galectin-13MAb (PP13/1161).
Immunohistochemistry-Paraffin: Galectin 13 Antibody (PP13/1161) [NBP2-53210] - Formalin-paraffin human Placenta stained with Galectin-13 MAb (PP13/1161).
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
BMP-4
[Unconjugated]
314-BP
1 ug/lane of Recombinant Human BMP-4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 22-25 kDa and 37-41 kDa, respectively. Multiple bands in gel are due to variable glycosylation. 
Recombinant Human BMP-4 (Catalog #314-BP/CF) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 2.5-15 ng/mL.
(8 Reviews)
(362 Publications)
Species: Hu
Placental
Lactogen/CSH1 Antibod ...
NBP2-46376
Western Blot: Placental Lactogen/CSH1 Antibody (2G4) [NBP2-46376] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSH1.
Immunohistochemistry: Placental Lactogen/CSH1 Antibody (2G4) [NBP2-46376] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Placental Lactogen/CSH1 Antibody (2G4) [NBP2-46376] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Species: Hu
Applications: WB, IHC
Chorionic
Gonadotropin beta Cha ...
NB100-63167B
Chorionic Gonadotropin beta Chain (hCG beta) Antibody (INN-hCG-53) [Biotin]
Species: Hu
Applications: WB, ELISA

Publications for hCG holo C2 Antibody (NB100-63167B) (0)

There are no publications for hCG holo C2 Antibody (NB100-63167B).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for hCG holo C2 Antibody (NB100-63167B) (0)

There are no reviews for hCG holo C2 Antibody (NB100-63167B). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for hCG holo C2 Antibody (NB100-63167B) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NB100-63167AF405
Alexa Fluor 488 NB100-63167AF488
Alexa Fluor 647 NB100-63167AF647
Alexa Fluor 700 NB100-63167AF700
Biotin NB100-63167B
DyLight 350 NB100-63167UV
DyLight 405 NB100-63167V
DyLight 488 NB100-63167G
DyLight 550 NB100-63167R
DyLight 650 NB100-63167C
DyLight 680 NB100-63167FR
DyLight 755 NB100-63167IR
FITC NB100-63167F
HRP NB100-63167H
Learn about Custom Antibody Labeling

Additional Chorionic Gonadotropin beta Chain (hCG beta) Products

Bioinformatics Tool for hCG holo C2 Antibody (NB100-63167B)

Discover related pathways, diseases and genes to hCG holo C2 Antibody (NB100-63167B). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for hCG holo C2 Antibody (NB100-63167B)

Discover more about diseases related to hCG holo C2 Antibody (NB100-63167B).
 

Pathways for hCG holo C2 Antibody (NB100-63167B)

View related products by pathway.

PTMs for hCG holo C2 Antibody (NB100-63167B)

Learn more about PTMs related to hCG holo C2 Antibody (NB100-63167B).
 

Research Areas for hCG holo C2 Antibody (NB100-63167B)

Find related products by research area.

Blogs on Chorionic Gonadotropin beta Chain (hCG beta)

There are no specific blogs for Chorionic Gonadotropin beta Chain (hCG beta), but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

1 ug/lane of Recombinant Human BMP-4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 22-25 kDa and 37-41 kDa, respectively. Multiple bands in gel are due to variable glycosylation. 
BMP-4 [Unconjugated]
314-BP-010

Contact Information

Learn the difference between western blot and simple western

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Chorionic Gonadotropin beta Chain (hCG beta) Antibody (INN-hCG-53) [Biotin] and receive a gift card or discount.


Bioinformatics

Gene Symbol CGB3
Entrez
Uniprot