Chorionic Gonadotropin beta Chain (hCG beta) Antibody (HCGab/52)

Immunohistochemistry-Paraffin: Chorionic Gonadotropin beta Chain (hCG beta) Antibody (HCGab/52) [NBP2-45334] - Human Placenta stained with hCG Monoclonal Antibody (HCGab/52).

• Reactivity: Hu
• Applications: IHC, IHC-P
• Clone: HCGab/52
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Chorionic Gonadotropin beta Chain (hCG beta) Antibody (HCGab/52) Summary

• Immunogen: Purified hCG protein
• Localization: Cytoplasmic, Secreted
• Marker: Pregnancy & Choriocarcinoma Marker
• Specificity: This MAb is a very special because it reacts ONLY with the intact-HCG and not with either free alpha- or free beta-chain of HCG. HCG is a glycoprotein and is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). HCG is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG MAb detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90% and 60% of cases respectively. 20% of lung squamous cell carcinomas are positive. HCG expression by non-trophoblastic tumors may indicate aggressive behavior.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CGB3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
• Application Notes: Hu-chromosome location: 6q12-q21 (HCG-alpha) & 19q13.33 (HCG-beta)
  Molecular weight of antigen: ~35kDa (intact hCG)
  Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Chorionic Gonadotropin beta Chain (hCG beta) Antibody (HCGab/52)

• CGB
• CGB3
• CGB3choriogonadotropin subunit beta
• CGB5
• CGB7
• CGB8
• CG-Beta
• Choriogonadotropin Subunit Beta
• Chorionic gonadotrophin chain beta
• chorionic gonadotropin beta 3 subunit
• Chorionic Gonadotropin beta Chain
• chorionic gonadotropin beta subunit
• chorionic gonadotropin, beta polypeptide
• hCGB

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CGB3