Home » Chorionic Gonadotropin alpha Chain (hCG alpha) » Chorionic Gonadotropin alpha Chain (hCG alpha) Antibodies » Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (INN-hCG-72) [DyLight 755]

Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (INN-hCG-72) [DyLight 755]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
INN-hCG-72
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 755

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NB100-62207AF405
Alexa Fluor 488
NB100-62207AF488
Alexa Fluor 647
NB100-62207AF647
Alexa Fluor 700
NB100-62207AF700
Biotin
NB100-62207B
DyLight 350
NB100-62207UV
DyLight 405
NB100-62207V
DyLight 488
NB100-62207G
DyLight 550
NB100-62207R
DyLight 650
NB100-62207C
DyLight 680
NB100-62207FR
FITC
NB100-62207F
HRP
NB100-62207H
Unconjugated
NB100-62207
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Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (INN-hCG-72) [DyLight 755] Summary

Immunogen
Human hCG
Specificity
This antibody recognises exclusively free hCG alpha. It reacts with the alpha 6 epitope on hCG alpha. No cross reaction is observed with holo-hCG in direct binding RIA of ELISA.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CGA
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (INN-hCG-72) [DyLight 755]

  • alpha HCG
  • CGA glycoprotein hormones, alpha polypeptide
  • CGA
  • CG-ALPHA
  • Chorionic Gonadotropin alpha Chain
  • FSHA
  • GPHa
  • GPHa1
  • HCG
  • LHA
  • TSHA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: PLOD1 Antibody [NBP2-38770] - SC65 directly interacts with lysyl-hydroxylase 1 (LH1). Western blot of primary calvarial osteoblast and skin fibroblast lysates from WT and Sc65KO 3 day-old mice (N = 2) showing significantly decreased levels of LH1 protein in Sc65KO samples. Densitometric quantification of LH1 protein normalized to beta-actin from the western blot shown above (*p<0.05; error bars represent SD). All experiments were performed at least 3 times. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pgen.1006002), licensed under a CC-BY licence.
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Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
Western blot shows lysates of HepG2 human hepatocellular carcinoma parental cell line and alpha ‑Fetoprotein/AFP knockout HepG2 cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated) in the parental HeLacell line, but is not detectable in knockout HeLa cell line. GAPDH(Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Applications: WB, Simple Western, CyTOF-ready, ICC, ICFlow, KO
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Western Blot: POMC Antibody [NB100-1533] - Staining of NIH3T3 cell lysate with antibody at 0.3 ug/mL (A) and Rat Brain cell lysate with antibody at 2 ug/mL (B). 35 ug protein in RIPA buffer. Detected by chemiluminescence.
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Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Immunohistochemistry of pancreas shows relatively higher reactivity for insulin in PrP-/- relative to Tg40 PrP sections (panels 1 & 3). Iron overloading decreases insulin reactivity in Tg40 PrP but not in PrP-/- samples (panels 1 vs. 2 & 3 vs. 4). Reaction for glucagon is higher in iron overloaded Tg40 PrP relative to control samples (panels 5 & 6). The difference is minimal in PrP-/- samples (panels 7 & 8). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
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Thyrotropin
Releasing Hormone Ant
NBP2-95244
Western Blot: Thyrotropin Releasing Hormone Antibody [NBP2-95244] - Analysis of extracts of various cell lines, using Thyrotropin Releasing Hormone at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry/Immunofluorescence: Thyrotropin Releasing Hormone Antibody [NBP2-95244] - Analysis of U2OS cells using Thyrotropin Releasing Hormone at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Thyrotropin Releasing Hormone Antibody [NBP2-95244] - Paraffin-embedded mouse brain using Thyrotropin Releasing Hormone.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC
PBX1 Antibody (4A2)
H00005087-M01
Western Blot: PBX1 Antibody (4A2) [H00005087-M01] - PBX1 monoclonal antibody (M01), clone 4A2 Analysis of PBX1 expression in Hela S3 NE.
Immunocytochemistry/Immunofluorescence: PBX1 Antibody (4A2) [H00005087-M01] - Analysis of monoclonal antibody to PBX1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: PBX1 Antibody (4A2) [H00005087-M01] - Analysis of monoclonal antibody to PBX1 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml.
(9 Publications)
Species: Hu, Mu, Pm
Applications: WB, ChIP, ELISA, ICC/IF, IHC, IHC-P, IP, S-ELISA

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NB100-62207AF405
Alexa Fluor 488 NB100-62207AF488
Alexa Fluor 647 NB100-62207AF647
Alexa Fluor 700 NB100-62207AF700
Biotin NB100-62207B
DyLight 350 NB100-62207UV
DyLight 405 NB100-62207V
DyLight 488 NB100-62207G
DyLight 550 NB100-62207R
DyLight 650 NB100-62207C
DyLight 680 NB100-62207FR
DyLight 755 NB100-62207IR
FITC NB100-62207F
HRP NB100-62207H
Learn about Custom Antibody Labeling

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