CHMP5 Antibody

Western Blot: CHMP5 Antibody [NB100-1512] - Staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CHMP5 Antibody Summary

• Immunogen: Peptide with sequence C-NKDGVLVDEFGLPQ corresponding to C-Terminus according to NP_057494.3.
• Predicted Species: Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CHMP5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 28 kDa band observed in K562 lysates is reported in scientific literature (PMID: 15644320).
• Control:

  CHMP5 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CHMP5 Antibody

• apoptosis-related protein PNAS-2
• C9orf83
• charged multivesicular body protein 5
• chromatin modifying protein 5
• Chromatin-modifying protein 5
• chromosome 9 open reading frame 83
• HSPC177
• hVps60
• PNAS-2
• SNF7 domain containing 2
• SNF7 domain-containing protein 2
• SNF7DC2
• Vacuolar protein sorting-associated protein 60
• Vps60CGI-34

Background

CHMP5 is a component of the ESCRT-III complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-III complex is probably involved in the concentration of MVB cargo. In case of infection, the HIV-1 virus takes advantage of the ESCRT-III complex for budding and exocytic cargoes of viral proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CHMP5 Antibody (NB100-1512)(1)

Publication using NB100-1512

• Ward DM, Vaughn MB, Shiflett SL et al. The role of LIP5 and CHMP5 in multivesicular body formation and HIV-1 budding in mammalian cells. J Biol Chem 2005-03-01 [PMID: 15644320]

Bioinformatics

• Gene Symbol: CHMP5
• Entrez:
  • 51510(Human)
• Uniprot:
  • NP_057494.2(Human)