CHMP5 Overexpression Lysate

Western Blot: CHMP5 Overexpression Lysate (Adult Normal) [NBL1-09162] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CHMP5.

• Reactivity: Hu
• Applications: WB

CHMP5 Overexpression Lysate Summary

• Description:

  CHMP5 Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC204779
  
  Accession#: NM_016410
  
  Protein Tag: C-MYC/DDK
• Gene: CHMP5

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 24.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CHMP5 Overexpression Lysate

• apoptosis-related protein PNAS-2
• C9orf83
• charged multivesicular body protein 5
• chromatin modifying protein 5
• Chromatin-modifying protein 5
• chromosome 9 open reading frame 83
• HSPC177
• hVps60
• PNAS-2
• SNF7 domain containing 2
• SNF7 domain-containing protein 2
• SNF7DC2
• Vacuolar protein sorting-associated protein 60
• Vps60CGI-34

Background

CHMP5 is a component of the ESCRT-III complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-III complex is probably involved in the concentration of MVB cargo. In case of infection, the HIV-1 virus takes advantage of the ESCRT-III complex for budding and exocytic cargoes of viral proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CHMP5