CHMP4A Antibody

Western Blot: CHMP4A Antibody [NBP3-06005] - Anti-CHMP4A rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: RT4 Whole Cell Lysate Lane C: HL60 Whole Cell Lysate Lane D: MOLT4 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)HRP at 110000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:25 kDa Observed band size:32 kDa

Immunohistochemistry-Paraffin: CHMP4A Antibody [NBP3-06005] - Staining of human CHMP4A in human rectum with rabbit polyclonal antibody at 1:100 dilution.

CHMP4A Antibody [NBP3-06005] - CHMP4A was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 4 uL anti-CHMP4A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG. Primary antibody: Anti-CHMP4A rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)HRP at 110000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 25 kDa Observed band size :32 kDa

Product Discontinued

CHMP4A Antibody Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human CHMP4A fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHMP4A
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1-5ul/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH7.0
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for CHMP4A Antibody

• C14orf123
• charged multivesicular body protein 4a
• CHMP4
• CHMP4a
• CHMP4B
• chromatin modifying protein 4A
• Chromatin-modifying protein 4a
• chromosome 14 open reading frame 123
• FLJ61658
• hSnf-1
• HSPC134
• hVps32-1
• MGC142093
• MGC142095
• SHAX2
• SNF7 homolog associated with Alix-2
• Snf7 homologue associated with Alix 2
• SNF7
• SNF7-1
• Vacuolar protein sorting-associated protein 32-1
• VPS32-1
• VPS32A

Background

CHMP4A (charged MVB protein 4A) is one of the three isoforms of CHMP4, the human equivalent of yeast Snf7 (so CHMP4A is also referred to as hSnf7-1). It is a component of the ESCRT-III (endosomal sorting complex required for transport III) complex. The ESCRT complexes mediate membrane budding away from the cytosol and cleavage of membrane necks (membrane fission) from the cytosolic face; hence, they are key in processes such as the formation of MVBs (multivesicular bodies), enveloped virus budding, and membrane abscission in cytokinesis. Not all types of ESCRT complexes, or proteins within those complexes, are necessary for all of these functions. For instance, research into the requirements of ESCRT-III proteins in HIV-1 budding has revealed that CHMP1 and CHMP3 proteins are not essential for this process; at least one member of each of the CHMP2 and CHMP4 families are required; within these families there is some functional redundancy; within the CHMP4 family, the relative importance and abundance of the isoforms is 4B > 4A > 4C; and the absence of CHMP4A or 4B prevents the budding process from progressing to completion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CHMP4A