Human Chitotriosidase/CHIT1 ELISA Kit (Colorimetric)

ELISA: Human Chitotriosidase/CHIT1 ELISA Kit (Colorimetric) [NBP2-75274] - Samples were spiked with high concentrations of Human Chitotriosidase/CHIT1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Chitotriosidase/CHIT1 ELISA Kit (Colorimetric) [NBP2-75274] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 78.13 - 5000 pg/mL
• Sensitivity: 46.88 pg/mL

Human Chitotriosidase/CHIT1 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human CHIT1 in samples. No significant cross-reactivity or interference between Human CHIT1 and analogues was observed.
• Standard Curve Range: 78.13 - 5000 pg/mL
• Sensitivity: 46.88 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.97%
• Intra-Assay: CV% < 5.04%
• Spike Recovery: 84-106%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CHIT1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Chitotriosidase/CHIT1 ELISA Kit (Colorimetric)

• CHI3
• CHIT1
• CHITCHI3
• chitinase 1 (chitotriosidase)
• chitinase-1
• Chitotriosidase
• chitotriosidase-1
• EC 3.2.1.14
• FLJ00314
• MGC125322
• plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase

Background

Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CHIT1