Chitotriosidase/CHIT1 Antibody [Alexa Fluor™ Plus 594]

• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor Plus 594

Chitotriosidase/CHIT1 Antibody [Alexa Fluor™ Plus 594] Summary

• Specificity: Detects mouse Chitotriosidase/CHIT1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross‑reactivity with recombinant human CHIT1 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Chitotriosidase, encoded by the mouse CHIT1 gene, is a typical member of the chitinase family (1). It is distinct from another member known as acidic mammalian chitinase (AMC), encoded by the CHIA gene, in several aspects. AMC/CHIA is expressed mainly in alveolar macrophages and in both the mouse and human gastrointestinal tract (2). CHIT1, however, is expressed exclusively by phagocytes in humans and in the gastrointestinal tract, tongue, fore-stomach, and Paneth cells of the small intestine in mice (2). Both CHIA and CHIT1 are secreted as 50 kDa proteins. In contrast to CHIA, CHIT1 is not stable under acidic pH and can be processed into a C-terminally truncated 39 kDa form (2‑4). Human CHIT1 is the best of three biomarkers in the monitoring of Gaucher disease (5). The other two commonly used markers include acid phosphatase and angiotensin-converting enzyme (ACE) (5). Human CHIT1 is also a specific marker of macrophage activation in acute ischemic stroke (6).

1. Zheng, T. et al. (2005) Gene 357:37.
2. Boot, R.G. et al. (2006) J. Histochem. Cytochem. 53:1283.
3. Boot, R.G. et al. (2001) J. Biol. Chem. 276:6770.
4. Renkema, G.H. et al. (1997) Eur. J. Biochem. 244:279.
5. Vellodi, A. et al. (2005) J. Inherit. Metab. Dis. 28:585.
6. Sotgiu, S. et al. (2005) Eur. Neurol. 54:149.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.