Chitotriosidase/CHIT1 Antibody (397513) [Alexa Fluor™ Plus 555]

• Reactivity: Hu
• Clone: 397513
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 555

Chitotriosidase/CHIT1 Antibody (397513) [Alexa Fluor™ Plus 555] Summary

• Specificity: Detects human Chitotriosidase/CHIT1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CHI3-L1 is observed.
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Chitotriosidase/CHIT1 Antibody (397513) [Alexa Fluor™ Plus 555]

• CHI3
• CHIT1
• CHITCHI3
• chitinase 1 (chitotriosidase)
• Chitinase-1
• Chitotriosidase
• chitotriosidase-1
• EC 3.2.1.14
• FLJ00314
• MGC125322
• plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase

Background

Chitotriosidase encoded by the CHIT1 gene is a member of the chitinase family that is selectively expressed in activated tissue macrophages (1). It is distinct from another member, known as acidic mammalian chitinase (AMC) encoded by the CHIA gene and expressed mainly in the gastrointestinal tract and lung (2). Both CHIA and CHIT1 are secreted as 50 kDa proteins. In contrast to CHIA, CHIT1 is not stable under acidic pHs and can be processed into a C-terminally truncated 39 kDa form (2, 3). CHIT1 is the best biomarker in the monitoring of Gaucher disease among the three most commonly used markers that also include acid phosphatase and angiotensin-converting enzyme (ACE) (4). CHIT1 is also a specific marker of macrophage activation in acute ischemic stroke (5).

1. Aguilera, B. et al. (2003) J. Biol. Chem. 278:40911.
2. Boot, R.G. et al. (2001) J. Biol. Chem. 276:6770.
3. Renkema, G.H. et al. (1997) Eur. J. Biochem. 244:279.
4. Vellodi, A. et al. (2005) J. Inherit. Metab. Dis. 28:585.
5. Sotgiu, S. et al. (2005) Eur. Neurol. 54:149.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.