CHES1 Overexpression Lysate (Native)

Images

 
Western Blot: CHES1 Overexpression Lysate (Adult Normal) [NBL1-10812] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CHES1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CHES1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201298 and based on accession number NM_005197. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens forkhead box N3 (FOXN3), transcript variant 2, mRNA.
Gene
FOXN3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
51.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for CHES1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CHES1 Overexpression Lysate (Native)

  • C14orf116
  • Checkpoint suppressor 1PRO1635
  • CHES1forkhead box protein N3
  • chromosome 14 open reading frame 116
  • forkhead box N3

Background

This gene is a member of the forkhead/winged helix transcription factor family. Checkpoints are eukaryotic DNA damage-inducible cell cycle arrests at G1 and G2. Checkpoint suppressor 1 suppresses multiple yeast checkpoint mutations including mec1, rad9, rad53 and dun1 by activating a MEC1-independent checkpoint pathway. Alternative splicing is observed at the locus, resulting in distinct isoforms. Transcript Variant: This variant (2) lacks an alternate in-frame exon and differs in the 5' UTR, compared to variant 1, resulting in a shorter protein (isoform 2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for CHES1 Lysate (NBL1-10812) (0)

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol FOXN3