CHD4 Antibody

Images

 
Western Blot: CHD4 Antibody [NB100-57520] - Detection of Human and Mouse CHD4/Mi2 beta on HeLa whole cell lysate using NB100-57520. CHD4/Mi2 beta was also IPed by rabbit anti-CHD4/Mi2 beta antibodies NB100-57519 and ...read more
Proximity Ligation Assay: CHD4 Antibody [NB100-57520] - image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

CHD4 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 250 and 300 of human chromodomain helicase DNA binding protein 4 (Mi2 autoantigen 218 kDa protein) using the numbering given in entry NP_001264.2 (GeneID 1108).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CHD4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 2 - 5 ug/mg lysate
  • Proximity Ligation Assay 1:200 - 1:4000
Publications
Read Publication using NB100-57520.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CHD4 Antibody

  • ATP-dependent helicase CHD4
  • CHD-4
  • chromodomain helicase DNA binding protein 4
  • chromodomain-helicase-DNA-binding protein 4
  • DKFZp686E06161
  • EC 3.6.1
  • EC 3.6.4.12
  • Mi-2 autoantigen 218 kDa protein
  • Mi-2b
  • Mi2-BETA

Background

CHD4/Mi2beta is a helicase component of the nucleosome remodeling and deacetylase (NuRD) complex that functions to remodel chromatin and repress transcription. Outside of the NuRD complex, CHD4/Mi2beta can function as an activator of transcription in association with p300 histone acetyltransferase. Alternate names for CHD4/Mi2beta include chromodomain-helicase-DNA-binding protein 4, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta, Mi-2b, and DKFZp686E06161.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CHD4 Antibody (NB100-57520)(1)

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CHD4
Entrez
Uniprot